Loss of VAPB Regulates Autophagy in a Beclin 1-Dependent Manner

Neuroscience Bulletin
Dan WuGuanghui Wang

Abstract

Autophagy is an evolutionarily-conserved self-degradative process that maintains cellular homeostasis by eliminating protein aggregates and damaged organelles. Recently, vesicle-associated membrane protein-associated protein B (VAPB), which is associated with the familial form of amyotrophic lateral sclerosis, has been shown to regulate autophagy. In the present study, we demonstrated that knockdown of VAPB induced the up-regulation of beclin 1 expression, which promoted LC3 (microtubule-associated protein light chain 3) conversion and the formation of LC3 puncta, whereas overexpression of VAPB inhibited these processes. The regulation of beclin 1 by VAPB was at the transcriptional level. Moreover, knockdown of VAPB increased autophagic flux, which promoted the degradation of the autophagy substrate p62 and neurodegenerative disease proteins. Our study provides evidence that the regulation of autophagy by VAPB is associated with the autophagy-initiating factor beclin 1.

References

Jun 21, 2007·The Journal of Biological Chemistry·Serhiy PankivTerje Johansen
Feb 29, 2008·Nature·Noboru MizushimaDaniel J Klionsky
Aug 15, 2008·Neurobiology of Aging·Georgia AnagnostouJacqueline de Belleroche
Jan 26, 2010·Current Opinion in Cell Biology·Congcong He, Beth Levine
Feb 11, 2010·Cell·Noboru MizushimaBeth Levine
Oct 14, 2010·The Journal of Biological Chemistry·Han-Jou ChenJackie S de Belleroche
Jan 21, 2011·Nature·Beth LevineHerbert W Virgin
Feb 12, 2011·Cell Death and Differentiation·R KangD Tang
Dec 2, 2011·Human Molecular Genetics·Kurt J De VosChristopher C J Miller
Apr 17, 2012·Cell·Mathieu Laplante, David M Sabatini
Nov 21, 2013·Acta Neuropathologica Communications·Marijn KuijpersDick Jaarsma
Dec 18, 2013·Cell Research·Ryan C RussellKun-Liang Guan
Jan 5, 2014·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Caitlin E O'Brien, Tony Wyss-Coray
Dec 7, 2014·Cell·Lorenzo GalluzziGuido Kroemer
Jul 30, 2015·Neuroscience Bulletin·Dongkai GuoGuanghui Wang
Jul 21, 2016·Molecular Pharmacology·Eduardo Cremonese Filippi-ChielaGuido Lenz
Nov 11, 2016·Nature·J Paul TaylorDon W Cleveland
Dec 30, 2016·Neuroscience Bulletin·Hong-Lei LiZhi-Ying Wu
Jan 31, 2017·Current Biology : CB·Patricia Gomez-SuagaChristopher C J Miller

❮ Previous
Next ❯

Citations

Apr 6, 2019·Neuroscience Bulletin·Zheng LiZixian Chen
Jan 25, 2019·Translational Neurodegeneration·Shen-Zhao LuJia-Wei Zhou
May 19, 2021·European Journal of Pharmacology·Zhenhui LuoQun Lu
Feb 8, 2022·Journal of Cell Science·Andrea MarkovinovicSebastien Paillusson

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
PCR
Fluorescence

Software Mentioned

GraphPad
Prism6
Photoshop

Related Concepts

Related Feeds

Autophagy & Disease

Autophagy is an important cellular process for normal physiology and both elevated and decreased levels of autophagy are associated with disease. Here is the latest research.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Amyloid Lateral Sclerosis

Amyotrophic Lateral Sclerosis (ALS) is a progressive nervous system disease associated with the death of neurons that control voluntary muscles. Discover the latest research on ALS here.

© 2022 Meta ULC. All rights reserved