Loureirin B Inhibits Hypertrophic Scar Formation via Inhibition of the TGF-β1-ERK/JNK Pathway

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Ting HeDahai Hu

Abstract

Our previous study confirmed that Loureirin B (LB) can inhibit hypertrophic scar formation. However, the mechanism of LB-mediated inhibition of scar formation is still unknown. Immunohistochemistry was used to detect expression of Col1, FN and TGF-β1 in skin and scar tissue. Fibroblasts were stimulated with TGF-β1 to mimic scar formation. LB or MAPK inhibitors were used to study the pathways involved in the process. Western blotting was used to evaluate the expression of p-JNK, p-ERK, p-p38, Col1 and FN. The contractile capacity of fibroblasts was evaluated using a gel contraction assay. Tissues were cultured ex vivo with LB to further investigate the participation of ERK and JNK in the LB-mediated inhibition of scar formation. FN and Col1 were up regulated in hypertrophic scars. LB down regulated p-ERK and p-JNK in TGF-β1-stimulated fibroblasts, while levels of phosphorylated p38 did not change. The down regulation of p-ERK and p-JNK was associated with a reduction of Col1 and FN. Similarly, inhibition of ERK and JNK down regulated the expression of Col1 and FN in TGF-β1-stimulated fibroblasts. LB down regulated protein levels of p-ERK and p-JNK in cultured hypertrophic scar tissue ex vivo. This study suggests that LB can inhi...Continue Reading

Citations

Apr 5, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yujuan LiWei Ren
Sep 11, 2019·Archives of Dermatological Research·Gil Soon ParkKwang Joong Kim
Dec 14, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Yu JiangTonghui Ma
Oct 1, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hui LiPeter C K Leung
Jul 20, 2019·Molecular Medicine Reports·Zheng ZuoMiansheng Zhu
Oct 14, 2020·The International Journal of Biochemistry & Cell Biology·Jun ShiYuyi Mai
Sep 22, 2020·Bioactive Materials·Lu ChenXiaoming Sun

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis