Lovastatin biosynthetic genes of Aspergillus terreus are expressed differentially in solid-state and in liquid submerged fermentation

Applied Microbiology and Biotechnology
Javier Barrios-GonzálezA Garay-Arroyo

Abstract

Molecular studies were performed to establish the causes of the superior lovastatin productivity of a novel solid-state fermentation (SSF) process, in relation with liquid submerged fermentation (SmF; 20 mg/g vs. 0.65 mg/ml). In SSF, biosynthetic genes lovE and lovF transcripts accumulated to high levels from day 1 to day 7. In this period, lovE transcript showed 4.6-fold higher accumulation levels (transcription) than the highest level detected in SmF (day 5). lovF transcript showed two-fold higher expression than the highest point in SmF. In SmF, the expression was only detected clearly on day 5 and, showing a 50% decrease, on day 7. These results show that the higher lovastatin production in SSF is related to a more intense transcription of these biosynthetic genes. A strong expression of gldB gene in lovastatin SSF indicated that Aspergillus terreus senses osmotic stress during the course of SSF, but not in SmF. However, when a liquid medium of identical concentration was used in SmF, lovastatin production decreased in SSF.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Aug 1, 1980·Developmental Biology·W E Timberlake
May 9, 2001·Applied Microbiology and Biotechnology·T RobinsonP Nigam
Jun 26, 2003·Molecular Microbiology·Ronald P de VriesJaap Visser
Feb 14, 2004·Applied Microbiology and Biotechnology·U HölkerJ Lenz
May 24, 2005·Applied Microbiology and Biotechnology·R te BiesebekeC A M J J van den Hondel
Jun 9, 2005·Current Opinion in Microbiology·Udo Hölker, Jürgen Lenz
Jul 8, 2005·Critical Reviews in Biotechnology·Chundakkadu Krishna
Oct 20, 2005·Journal of Bioscience and Bioengineering·M DomínguezJ Barrios-González
May 9, 2006·Journal of Industrial Microbiology & Biotechnology·Ramunas BigelisMichael Greenstein

❮ Previous
Next ❯

Citations

Oct 13, 2009·Applied Microbiology and Biotechnology·Javier Barrios-González, Roxana U Miranda
Jun 24, 2010·Applied Microbiology and Biotechnology·Flavia SorrentinoTajalli Keshavarz
Dec 23, 2008·Applied Biochemistry and Biotechnology·N S ShaligramG Szakacs
Aug 6, 2013·Applied Biochemistry and Biotechnology·Sudheer Kumar Singh, Ashok Pandey
Apr 15, 2015·Biotechnology Advances·Kelly C L MulderNádia S Parachin
Mar 10, 2010·Critical Reviews in Microbiology·Beatriz RuizElizabeth Langley
Jul 25, 2019·Current Pharmaceutical Biotechnology·Roberval N M NetoRita de C M de Miranda
Aug 10, 2017·Applied Microbiology and Biotechnology·Ailed Pérez-SánchezJavier Barrios-González
Apr 30, 2016·Journal of Fungi·Mishal SubhanIan Macreadie
Sep 16, 2020·Applied Microbiology and Biotechnology·Javier Barrios-GonzálezMaría Esmeralda Bibián
May 14, 2020·Applied Biochemistry and Biotechnology·Victor Contreras-JácquezAli Asaff-Torres
Sep 5, 2020·Chembiochem : a European Journal of Chemical Biology·Eiichiro KanYasuo Ohnishi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.