Lovastatin inhibits the stimulation of mitogen-activated protein kinase by insulin in HIRcB fibroblasts

Archives of Biochemistry and Biophysics
X Q XuG Romero

Abstract

Lovastatin, a cholesterol biosynthesis inhibitor, has recently been shown to inhibit mitogenesis and tumor growth. We have investigated the effects of lovastatin on the activation of MAP kinase by insulin using as a model HIRcB cells, a rat fibroblast cell line that overexpresses the human insulin receptor. Treatment with lovastatin (1-30 microM) for 24 h decreased the level of activation of MAP kinase by insulin by as much as 60%. Immunoblotting experiments using a specific anti-MAP kinase monoclonal antibody demonstrated that the amount of MAP kinase protein in the cells was not altered by lovastatin treatment. Likewise, lovastatin had no apparent effects on the expression of the insulin receptor. Treatment with lovastatin (20 microM) reduced the percentage of farnesylated Ras by 50%. Immunoprecipitation of tyrosine phosphorylated proteins from HIRcB cell lysates followed by immunodetection of MAP kinase using specific antibodies demonstrated a reduced level of insulin-induced tyrosine phosphorylation levels of MAP kinase in lovastatin-treated cells. Furthermore, immunodetection of the beta-subunit of the insulin receptor in anti-phosphotyrosine immunoprecipitates revealed that treatment with lovastatin reduced the tyrosine p...Continue Reading

Citations

Oct 6, 2015·Expert Opinion on Drug Safety·Aris P AgouridisMoses S Elisaf
Feb 10, 2016·Drugs·Matthew H DavenportCraig A Erickson
Sep 13, 2011·Pediatric Neurology·Maria T AcostaRoger J Packer
Sep 27, 2014·American Journal of Medical Genetics. Part a·Artuela ÇakuFrancois Corbin
Oct 21, 2006·International Immunopharmacology·Antonio G Tristano, Kathy Fuller
Sep 28, 2016·Intractable & Rare Diseases Research·Andrew Ligsay, Randi J Hagerman
Oct 7, 2003·Vascular and Endovascular Surgery·Daphne Pierre-Paul, Vivian Gahtan
Sep 2, 1999·International Journal of Cancer. Journal International Du Cancer·G P PrevostB Morgan
Jun 27, 2019·Current Pediatric Reviews·Dragana ProticRandi J Hagerman
Sep 16, 2015·The Journal of Biological Chemistry·Kenji FukuiC Ronald Kahn
Jun 16, 2017·Journal of Neurodevelopmental Disorders·Craig A EricksonElizabeth Berry-Kravis
Nov 21, 2020·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Maria Jimena Salcedo-ArellanoRandi J Hagerman

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