Low aspect ratio micropores for single-particle and single-cell analysis

Electrophoresis
Gaurav GoyalMin Jun Kim

Abstract

This paper describes microparticle and bacterial translocation studies using low aspect ratio solid-state micropores. Micropores, 5 μm in diameter, were fabricated in 200 nm thick free-standing silicon nitride membranes, resulting in pores with an extremely low aspect ratio, nominally 0.04. For microparticle translocation experiments, sulfonated polystyrene microparticles and magnetic microbeads in size range of 1-4 μm were used. Using the microparticle translocation characteristics, we find that particle translocations result in a change only in the pore's geometrical resistance while the access resistance remains constant. Furthermore, we demonstrate the ability of our micropore to probe high-resolution shape information of translocating analytes using concatenated magnetic microspheres. Distinct current drop peaks were observed for each microsphere of the multibead architecture. For bacterial translocation experiments, nonflagellated Escherichia coli (strain HCB 5) and wild type flagellated Salmonella typhimurium (strain SJW1103) were used. Distinct current signatures for the two bacteria were obtained and this difference in translocation behavior was attributed to different surface protein distributions on the bacteria. Our...Continue Reading

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Citations

Nov 9, 2016·Nanotechnology·Gaurav GoyalMin Jun Kim
Aug 29, 2018·Electrophoresis·Armin DarvishMin Jun Kim
Nov 20, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Taisuke ShimadaYoshinobu Baba
Dec 14, 2017·Scientific Reports·Makusu TsutsuiTomoji Kawai
Mar 18, 2017·Nanotechnology·Sou RyuzakiTomoji Kawai
Sep 8, 2017·Journal of the American Chemical Society·Hirotoshi YasakiYoshinobu Baba

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