Low background FRET-substrates for lipases and esterases suitable for high-throughput screening under basic (pH 11) conditions

Organic & Biomolecular Chemistry
Yongzheng YangJean-Louis Reymond

Abstract

FRET-based fluorogenic substrates for lipases and esterases were prepared in four steps from commercially available building blocks. The substrates are pyrenebutyric acid monoesters of aliphatic 1,2-diols bearing a dinitrophenylamino group as a quencher. The most enzyme-reactive substrate is ester 2a. The substrates do not show any measurable background reaction in the absence of enzyme even at pH 11, but react fast and specifically with lipases and esterases. These substrates offer an unprecedented and practical solution to the long-standing problem of a simple yet efficient high-throughput screening tool for lipase activities under basic conditions.

References

Dec 1, 1969·Analytical Chemistry·G G Guilbault, J Hieserman
Apr 3, 2001·Current Opinion in Chemical Biology·D Wahler, J L Reymond
Feb 19, 2002·Current Opinion in Biotechnology·D Wahler, J L Reymond
Jun 1, 2002·Current Opinion in Chemical Biology·Manfred T Reetz
Aug 31, 2002·Chembiochem : a European Journal of Chemical Biology·Jean-Louis Reymond, Denis Wahler
Nov 30, 2002·Angewandte Chemie·Hening Lin, Virginia W Cornish
Dec 20, 2002·Angewandte Chemie·Denis Wahler, Jean-Louis Reymond
Jun 3, 2004·Chembiochem : a European Journal of Chemical Biology·Johann Grognux, Jean-Louis Reymond
Jul 13, 2004·Trends in Biotechnology·Jean-Philippe Goddard, Jean-Louis Reymond
Sep 10, 2004·Journal of the American Chemical Society·Jean-Philippe Goddard, Jean-Louis Reymond
Sep 11, 2004·Current Opinion in Biotechnology·Jean-Philippe Goddard, Jean-Louis Reymond
Jan 15, 2005·Analytical Chemistry·Peter Babiak, Jean-Louis Reymond
Apr 29, 2005·Biomolecular Engineering·Marlen Schmidt, Uwe T Bornscheuer
May 17, 2005·Methods : a Companion to Methods in Enzymology·Dean Gilham, Richard Lehner
Aug 2, 2006·Molecular BioSystems·Yang Yongzheng, Jean-Louis Reymond
Jul 3, 1998·Angewandte Chemie·Rolf D Schmid, Robert Verger

❮ Previous
Next ❯

Citations

May 8, 2010·Chemical Communications : Chem Comm·Nan DaiEric T Kool
Dec 22, 2007·Annals of the New York Academy of Sciences·Jean-Louis Reymond
Jul 4, 2008·Annals of the New York Academy of Sciences·Corinna M SpanglerMichael Schäerling
Jun 1, 2016·Chemistry and Physics of Lipids·Rokhsana J Andersen, Jesper Brask
Jan 12, 2007·Molecular BioSystems·Johann Grognux, Jean-Louis Reymond
Dec 22, 2006·Biotechnology Journal·Zhen QianStefan Lutz
Jan 16, 2007·Biotechnology Journal·Renaud SicartJean-Louis Reymond
Dec 17, 2008·Chemical Communications : Chem Comm·Jean-Louis ReymondNoélie Maillard
May 7, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hongje JangDal-Hee Min
Dec 15, 2015·Beilstein Journal of Organic Chemistry·Raphael Reuter, Thomas R Ward
Jun 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Meng GaoBin Liu
Oct 9, 2021·Chemistry : a European Journal·Dihua DaiYing-Wei Yang
Jun 29, 2012·Bioorganic & Medicinal Chemistry Letters·Meghan JohnstonRichard I Duclos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.