Low bacterial community diversity in two introduced aphid pests revealed with 16S rRNA amplicon sequencing

PeerJ
Francisca Zepeda-PauloBlas Lavandero

Abstract

Bacterial endosymbionts that produce important phenotypic effects on their hosts are common among plant sap-sucking insects. Aphids have become a model system of insect-symbiont interactions. However, endosymbiont research has focused on a few aphid species, making it necessary to make greater efforts to other aphid species through different regions, in order to have a better understanding of the role of endosymbionts in aphids as a group. Aphid endosymbionts have frequently been studied by PCR-based techniques, using species-specific primers, nevertheless this approach may omit other non-target bacteria cohabiting a particular host species. Advances in high-throughput sequencing technologies are complementing our knowledge of microbial communities by allowing us the study of whole microbiome of different organisms. We used a 16S rRNA amplicon sequencing approach to study the microbiome of aphids in order to describe the bacterial community diversity in introduced populations of the cereal aphids, Sitobion avenae and Rhopalosiphum padi in Chile (South America). An absence of secondary endosymbionts and two common secondary endosymbionts of aphids were found in the aphids R. padi and S. avenae, respectively. Of those endosymbion...Continue Reading

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Citations

Apr 7, 2019·GigaScience·Wenbo ChenGeorg Jander
Mar 27, 2021·Evolutionary Applications·Laura BankersKayla C King
May 29, 2021·Evolution; International Journal of Organic Evolution·Çiğdem ÜnJan Oettler
Nov 28, 2021·Molecular Ecology·Antonino Malacrinò

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Datasets Mentioned

BETA
MG958612
MG958613
MG958614
MF536106

Methods Mentioned

BETA
amplicon sequencing
electrophoresis
PCR
Assay
Illumina sequencing

Software Mentioned

Geneious
QIIME
PANDAseq
PRINSEQ
Trimmomatic

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