Low cardiorespiratory fitness is associated with higher extracellular vesicle counts in obese adults

Physiological Reports
Natalie Z M EichnerSteven K Malin

Abstract

Low cardiorespiratory fitness (CRF) is associated with cardiovascular disease (CVD) independent of obesity. Extracellular vesicles (EVs) are a novel target of CVD, however, it remains unknown if obese individuals with very poor fitness (VPF) have elevated EVs versus people with poor fitness (PF). Thus, we tested whether VPF was associated with greater EV subtypes in obese adults. Subjects with VPF (n = 13, VO2 peak: 15.4 ± 0.6 mL/kg/min, BMI: 34.1 ± 1.7 kg/m2 ) and PF (n = 13, VO2 peak: 25.9 ± 3.0 mL/kg/min, BMI: 32.1 ± 1.2 kg/m2 ) were compared in this cross-sectional study. After an overnight fast, AnnexinV (AV) +/- platelet (CD31+ /CD41+ ), leukocyte (CD45+ /CD41- ), and endothelial EVs (CD105+ , CD31+ /CD41- ) were analyzed from fresh platelet poor plasma via imaging flow cytometry. Body fat, blood pressure (BP), and glucose tolerance (OGTT) were also tested. Body weight, BP, and circulating glucose were similar between groups, although VPF subjects were older than PF (64.0 ± 2.1 vs. 49.8 ± 4.2 year; P < 0.05). People with VPF, compared with PF, had higher total AV- EVs (P = 0.04), AV- platelet EVs (CD31+ /CD41+ ; P = 0.006), and AV- endothelial EVs (CD31+ /CD41- ; P = 0.005) independent of age and body fat. Higher AV- plat...Continue Reading

References

Dec 1, 1996·Journal of the American College of Cardiology·J Niebauer, J P Cooke
Nov 5, 1999·JAMA : the Journal of the American Medical Association·M WeiS N Blair
Jul 24, 2002·Circulation·Michael J LaMonteBarbara E Ainsworth
Feb 8, 2003·Hypertension·Richard A PrestonYeon S Ahn
Sep 24, 2004·JAMA : the Journal of the American Medical Association·Katherine EspositoDario Giugliano
Dec 1, 2004·Circulation·Alexandre C FerreiraEduardo de Marchena
Apr 22, 2005·The New England Journal of Medicine·Göran K Hansson
Mar 23, 2006·JAMA : the Journal of the American Medical Association·Samia MoraPaul M Ridker
May 27, 2006·Journal of Applied Physiology·Paul J MillsAlan S Maisel
Sep 2, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Matteo PirroElmo Mannarino
Oct 14, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Gilles ChironiAlain Tedgui
Oct 11, 2008·Medicine and Science in Sports and Exercise·Brian A IrvingArthur Weltman
May 21, 2009·JAMA : the Journal of the American Medical Association·Satoru KodamaHirohito Sone
Jun 18, 2009·Current Diabetes Reviews·Shosaku Nomura
Jan 7, 2010·Current Opinion in Nephrology and Hypertension·Chantal M Boulanger
May 18, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Nastassia M NavasiolavaMarc-Antoine Custaud
Nov 12, 2010·Seminars in Thrombosis and Hemostasis·Nicolas AmabileChantal M Boulanger
Dec 17, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Françoise Dignat-George, Chantal M Boulanger
Mar 24, 2011·Journal of Cardiopulmonary Rehabilitation and Prevention·Carl J LavieConrad P Earnest
Oct 4, 2011·Vascular Health and Risk Management·Michael D BrownJoon-Young Park
Dec 1, 2012·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Saurabh S ThosarJanet P Wallace
Apr 26, 2013·Circulation. Heart Failure·Paul A HeidenreichUNKNOWN Stroke Council
Sep 21, 2013·American Journal of Physiology. Endocrinology and Metabolism·Steven K MalinJohn P Kirwan
Mar 13, 2014·Journal of Thrombosis and Haemostasis : JTH·N ArraudA R Brisson
Jun 7, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Uta ErdbrüggerJoanne Lannigan
Feb 11, 2015·Pediatrics·Luc BruyndonckxViviane M Conraads
May 13, 2015·Biology of Sex Differences·Callie M GustafsonMuthuvel Jayachandran
Oct 11, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Emeline M Van CraenenbroeckVicky Y Hoymans
Oct 22, 2015·Experimental Physiology·Kasey A LansfordNathan T Jenkins
Dec 10, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Uta Erdbrügger, Joanne Lannigan
Mar 1, 2017·Nature Methods·UNKNOWN EV-TRACK ConsortiumAn Hendrix
Sep 21, 2017·Cardiovascular Diabetology·Alessandra GiannellaGiulio Ceolotto

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Citations

Jan 11, 2019·Journal of Applied Physiology·Emily M HeistonSteven K Malin
Nov 7, 2019·Diabetologia·Naveed AkbarMyriam Aouadi
Oct 15, 2019·Medicine and Science in Sports and Exercise·Natalie Z M EichnerSteven K Malin
Nov 30, 2019·Frontiers in Pharmacology·Francesco TausPietro Minuz

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Methods Mentioned

BETA
flow cytometry
scanning electron microscopy
ubiquitination

Software Mentioned

Particle Metrix
FCS Express6 DeNovo
Zetaview
Izon Control Suite
EV
IDEAS
ISX

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