Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells

Stem Cell Reports
Leqian YuLi Liu

Abstract

We show that a human pluripotent stem cell (hPSC) population cultured on a low-adhesion substrate developed two hPSC subtypes with different colony morphologies: flat and domed. Notably, the dome-like cells showed higher active proliferation capacity and increased several pluripotent genes' expression compared with the flat monolayer cells. We further demonstrated that cell-matrix adhesion mediates the interaction between cell morphology and expression of KLF4 and KLF5 through a serum response factor (SRF)-based regulatory double loop. Our results provide a mechanistic view on the coupling among adhesion, stem cell morphology, and pluripotency, shedding light on the critical role of cell-matrix adhesion in the induction and maintenance of hPSC.

References

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Citations

Jun 21, 2020·International Journal of Molecular Sciences·Shu-Wei HuangFeng-Huei Lin
Dec 15, 2019·Scientific Reports·Theresa BenteleMotomu Tanaka
Feb 19, 2019·Frontiers in Chemistry·Hiroki SakutaKenichi Yoshikawa
Sep 11, 2018·Stem Cell Reports·Takahisa MatsuzakiTakanori Takebe
Mar 23, 2021·ACS Biomaterials Science & Engineering·Anusuya RamasubramanianDavid V Schaffer
Oct 20, 2020·Stem Cells·Xiao HuShangqin Guo

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Methods Mentioned

BETA
enzymatic dissociation
PCR
transfection
RNA-seq

Software Mentioned

MATLAB
ImageJ
R
DAVID

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