PMID: 3768300Aug 12, 1986Paper

Low concentrations of bile salts increase the rate of spontaneous phospholipid transfer between vesicles

Biochemistry
J W Nichols

Abstract

The rate of 1-palmitoyl-2-[12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino] dodecanoyl] phosphatidylcholine (P-C12-NBD-PC) transfer between dioleoylphosphatidylcholine vesicles was measured by a technique based on resonance energy transfer between P-C12-NBD-PC and N-(lissamine rhodamine B sulfonyl)dioleoylphosphatidylethanolamine [Nichols, J. W., & Pagano, R. E. (1982) Biochemistry 21, 1720-1726]. Addition of bile salts at concentrations below their critical micelle concentrations increased the rate of spontaneous P-C12-NBD-PC transfer without disrupting the vesicles. The effectiveness in increasing the transfer rate was dependent on the structure of the bile salt. In general, conjugated bile salts were more effective than unconjugated, and mono- and dihydroxy bile salts were more effective than trihydroxy. The kinetics of intervesicular P-C12-NBD-PC transfer in the presence of cholate were found to be consistent with a mass action kinetic model based on the premise that bile salts bind to the vesicles, alter the dissociation and/or association rate constants for phospholipid monomer-vesicle interaction, and increase the rate of phospholipid transfer via the diffusion of soluble monomers through the aqueous phase. Temperature depen...Continue Reading

References

Sep 19, 1977·Biochimica Et Biophysica Acta·G Duckwitz-peterleinP Overath
Sep 19, 1977·Biochimica Et Biophysica Acta·L Thilo
Aug 23, 1977·Biochemistry·J M KremerP H Wiersema
Nov 1, 1969·Journal of Colloid and Interface Science·M C Carey, D M Small
Jan 18, 1983·Biochemistry·M De CuyperH Dangreau
Feb 5, 1980·Biochemistry·M A Roseman, T E Thompson
May 12, 1981·Biochemistry·J W Nichols, R E Pagano
Jul 7, 1981·Biochemistry·D K StruckR E Pagano
Sep 1, 1981·Steroids·W T BeherJ Sanfield

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Citations

Mar 21, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J M Donovan, A A Jackson
May 1, 1995·Annals of Biomedical Engineering·D Needham, D V Zhelev
Jun 24, 1987·Biochimica Et Biophysica Acta·M C PhillipsG H Rothblat
Oct 7, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Monalisa Mohapatra, Ashok K Mishra
Sep 1, 1995·Photochemistry and Photobiology·C F ChapmanB J Tromberg
Oct 27, 2011·International Journal of Pharmaceutics·Sarah Maud FischerMartin Brandl
Apr 4, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Elenaz NaderkhaniGøril Eide Flaten
Aug 18, 2001·Biophysical Journal·K TanhuanpääP Somerharju
May 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P PodevinG Cherqui
Oct 30, 2013·The Journal of Biological Chemistry·Yong ZhouIlya Levental

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