PMID: 7537471Apr 1, 1995Paper

Low-conductance anion channel activated by cAMP in teleost Cl- -secreting cells

The American Journal of Physiology
W S MarshallW F Hamilton

Abstract

We studied characteristics and modulation of ion channels in primary cultures of opercular epithelium from the euryhaline marine killifish Fundulus heteroclitus. Primary cultures, 17-28 h old, retain mitochondria-rich Cl- cells identifiable by fluorescence microscopy. Cell-attached patches revealed frequent low-conductance 8.1 +/- 0.35 pS channels that usually became inactive on excision; high-conductance anion channels were not apparent. Ion substitution experiments demonstrated selectivity for Cl- over gluconate of 1:0.07. With addition of 1-isobutyl-3-methylxanthine (0.1 mM) and dibutyryladenosine 3',5'-cyclic monophosphate (1.0 mM) to the bath, incidence of the channel increased from 35.3 to 61.9% of total patches (n = 156 and 21, respectively), and incidence of patches with multiple copies of the channel increased markedly from 2.2 to 38.5%. Epithelial Cl- transport was inhibited by mucosally added diphenylamine-2-carboxylic acid (1.0 mM) but not by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (0.1-1.0 mM). The anion channel was absent from cultured killifish corneal epithelium, a tissue that lacks Cl- cells. We conclude that a low-conductance anion channel of Cl- cells, likely in the apical membrane, may account for a...Continue Reading

Citations

Jan 30, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Martine AvellaPhilippe Poujeol
Sep 24, 2011·American Journal of Physiology. Cell Physiology·Maximilian StahlJohn N Forrest
Nov 22, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yusuke KumaiSteve F Perry
Oct 28, 2015·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Richard T Di Giulio, Bryan W Clark
Jun 6, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·David H Evans
Jun 21, 2006·The Journal of Comparative Neurology·Michael G Jonz, Colin A Nurse
Sep 18, 2020·The Journal of Experimental Biology·Stephen D McCormickAmy M Regish
Dec 1, 2009·The Journal of Experimental Biology·S D McCormickA K Christensen
Mar 10, 2015·The Journal of Experimental Biology·Regina R F CozziWilliam S Marshall

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