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Low-cost, low-input RNA-seq protocols perform nearly as well as high-input protocols

PeerJ

Apr 4, 2015

Peter A Combs, Michael B Eisen

Abstract

Recently, a number of protocols extending RNA-sequencing to the single-cell regime have been published. However, we were concerned that the additional steps to deal with such minute quantities of input sample would introduce serious biases that would make analysis of the data using exis...read more

Mentioned in this Paper

Embryo
Study
Sequence Determinations, RNA
Entire Embryo
Transcription, Genetic
Ultrasound, Transrectal
Drosophila
Gene Amplification Technique
Gene Expression
Evaluation
49
1
148
Paper Details
References
  • References28
  • Citations6
123
  • References28
  • Citations6
1

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Low-cost, low-input RNA-seq protocols perform nearly as well as high-input protocols

PeerJ

Apr 4, 2015

Peter A Combs, Michael B Eisen

PMID: 25834775

DOI: 10.7717/peerj.869

Abstract

Recently, a number of protocols extending RNA-sequencing to the single-cell regime have been published. However, we were concerned that the additional steps to deal with such minute quantities of input sample would introduce serious biases that would make analysis of the data using exis...read more

Mentioned in this Paper

Embryo
Study
Sequence Determinations, RNA
Entire Embryo
Transcription, Genetic
Ultrasound, Transrectal
Drosophila
Gene Amplification Technique
Gene Expression
Evaluation
49
1
148

Trending Feeds

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Related Papers

Nature Biotechnology

How deep is enough in single-cell RNA-seq?

Nature BiotechnologyOctober 10, 2014
Aaron M Streets, Yanyi Huang
Nature Genetics

High-input biology

Nature GeneticsSeptember 1, 2005
Paper Details
References
  • References28
  • Citations6
123
  • References28
  • Citations6
1
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