Low-cost microphysiological systems: feasibility study of a tape-based barrier-on-chip for small intestine modeling.

Lab on a Chip
Thomas E WinklerAnna Herland

Abstract

We see affordability as a key challenge in making organs-on-chips accessible to a wider range of users, particularly outside the highest-resource environments. Here, we present an approach to barrier-on-a-chip fabrication based on double-sided pressure-sensitive adhesive tape and off-the-shelf polycarbonate. Besides a low materials cost, common also to PDMS or thermoplastics, it requires minimal (€100) investment in laboratory equipment, yet at the same time is suitable for upscaling to industrial roll-to-roll manufacture. We evaluate our microphysiological system with an epithelial (Caco-2/BBe1) barrier model of the small intestine, studying the biological effects of permeable support pore size, as well as stimulation with a common food compound (chili pepper-derived capsaicinoids). The cells form tight and continuous barrier layers inside our systems, with comparable permeability but superior epithelial polarization compared to Transwell culture, in line with other perfused microphysiological models. Permeable support pore size is shown to weakly impact barrier layer integrity as well as the metabolic cell profile. Capsaicinoid response proves distinct between culture systems, but we show that impacted metabolic pathways are ...Continue Reading

References

Nov 1, 1992·General Pharmacology·G B Glavin
Apr 4, 1998·The Journal of Nutrition·E Jensen-JarolimJ Graf
Apr 29, 1998·Annual Review of Physiology·J L Madara
Jan 6, 1999·Journal of Pharmaceutical Sciences·J D IrvineJ R Grove
Jan 11, 2005·Archives of Biochemistry and Biophysics·R PrabhuK A Balasubramanian
May 18, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Roula Konsoula, Frank A Barile
Apr 15, 2006·Digestive Diseases and Sciences·Prabhu RamamoorthyKunissery A Balasubramanian
Jan 5, 2007·Lab on a Chip·Michael W Toepke, David J Beebe
Oct 6, 2007·Biological & Pharmaceutical Bulletin·Yuri TsukuraKazuhiko Tanaka
Jan 3, 2008·The Journal of Physiology·Michael J Joyner, Bengt Saltin
Mar 28, 2008·American Journal of Physiology. Cell Physiology·Elbert L LeeYasunobu Okada
Oct 28, 2008·Biochemical and Biophysical Research Communications·Yoko NagumoToshiyuki Tanaka
Nov 13, 2008·Cytotechnology·H IsodaT Maekawa
Feb 22, 2012·Medical Image Analysis·Pierrick CoupéD Louis Collins
Apr 27, 2012·Analytical Chemistry·Ralf TautenhahnGary Siuzdak
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Oct 12, 2012·Nature Biotechnology·Matthew C ChambersParag Mallick
Nov 8, 2012·Journal of Neuroscience Methods·Diane M WuestKelvin H Lee
Jul 3, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Hyun Jung Kim, Donald E Ingber
Aug 6, 2014·Nature Biotechnology·Sangeeta N Bhatia, Donald E Ingber
Feb 13, 2015·Critical Reviews in Food Science and Nutrition·Krishnapura Srinivasan
Apr 19, 2015·BMC Bioinformatics·Gunnar LibisellerChristoph Magnes
Dec 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Hyun Jung KimDonald E Ingber
Feb 13, 2016·Journal of Pharmaceutical Sciences·Jenni KüblbeckPaavo Honkakoski
May 4, 2016·Tissue Barriers·Marinke W van der HelmLoes I Segerink
May 16, 2018·Nucleic Acids Research·Jasmine ChongJianguo Xia
May 19, 2018·Nutrients·Sunil K PanchalLindsay Brown
Mar 27, 2019·Metabolites·Jasmine ChongJianguo Xia
Apr 7, 2019·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Joan CabellosGemma Janer
Jun 5, 2019·International Journal of Pharmaceutics·S HinkelC C Müller-Goymann
Jun 7, 2019·Lab on a Chip·Iago PereiroGovind V Kaigala

❮ Previous
Next ❯

Methods Mentioned

BETA
chip
chips
PCA
aminoacylation

Software Mentioned

ARcare
TissUse
LabView
MetaboAnalyst
mummichog
Emulate
XCMS CAMERA
statTarget
ProteoWizard
GSEA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.