Low-cost simultaneous detection of CCR5-delta32 and HLA-B*5701 alleles in human immunodeficiency virus type 1 infected patients by selective multiplex endpoint PCR

Journal of Virological Methods
Andrea RosiMaurizio Zazzi

Abstract

Host genetic traits impact susceptibility to human immunodeficiency virus type 1 (HIV-1) infection, disease progression as well as antiretroviral drug pharmacokinetics and toxicity. Remarkable examples include a 32-bp deletion in the CCR5 coreceptor molecule (CCR5-delta32) impairing attachment of monocytotropic HIV-1 to the host cell membrane and the HLA-B*5701 allele, strongly associated with a potentially fatal hypersensitivity reaction triggered by abacavir, a nucleoside inhibitor of HIV reverse transcriptase. We developed a simple selective multiplex endpoint PCR method for simultaneous analysis of both genetic traits. Two primers were designed for amplification of a region surrounding the CCR5 32-bp deletion site. One common forward primer and two reverse primers with different 3' termini targeting the HLA-B*570101 and HLA-B*570102 alleles were designed for HLA-B*5701 analysis. A panel of 110 reference DNA samples typed in the HLA-B locus was used for development and blind validation of the assay. All the 45 HLA-B*5701 positive and the 55 HLA-B*5701 negative samples were correctly identified. The CCR5-delta32 allele was readily detected in 7 samples and did not interfere with detection of HLA-B*5701 while providing an inte...Continue Reading

References

Sep 19, 2006·Seminars in Immunology·Fernando Arenzana-Seisdedos, Marc Parmentier
Dec 31, 2009·PLoS Genetics·Jacques FellayUNKNOWN NIAID Center for HIV/AIDS Vaccine Immunology (CHAVI)
Jun 12, 2013·Frontiers in Immunology·Sophie Limou, Jean-François Zagury
Aug 8, 2013·AIDS·UNKNOWN UK Collaborative HIV Cohort Study Steering Committee
Nov 20, 2013·Retrovirology·Sagar A VaidyaMarcus Altfeld
Apr 26, 2014·International Journal of Immunogenetics·C Darke, S A Corbin
Jun 19, 2014·Expert Opinion on Drug Metabolism & Toxicology·Pablo BarreiroVincent Soriano
May 3, 2015·The Journal of Allergy and Clinical Immunology·Bernardo Sousa-PintoLuís Delgado

❮ Previous
Next ❯

Citations

Mar 8, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Genny MeiniMaurizio Zazzi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.