Low-cost solution for rodent home-cage behaviour monitoring

PloS One
Surjeet SinghMajid Mohajerani

Abstract

In the current research on measuring complex behaviours/phenotyping in rodents, most of the experimental design requires the experimenter to remove the animal from its home-cage environment and place it in an unfamiliar apparatus (novel environment). This interaction may influence behaviour, general well-being, and the metabolism of the animal, affecting the phenotypic outcome even if the data collection method is automated. Most of the commercially available solutions for home-cage monitoring are expensive and usually lack the flexibility to be incorporated with existing home-cages. Here we present a low-cost solution for monitoring home-cage behaviour of rodents that can be easily incorporated to practically any available rodent home-cage. To demonstrate the use of our system, we reliably predict the sleep/wake state of mice in their home-cage using only video. We validate these results using hippocampal local field potential (LFP) and electromyography (EMG) data. Our approach provides a low-cost flexible methodology for high-throughput studies of sleep, circadian rhythm and rodent behaviour with minimal experimenter interference.

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Citations

Aug 20, 2020·Current Protocols in Mouse Biology·Liane HobsonPatrick M Nolan
Nov 18, 2019·Experimental Brain Research·Surjeet SinghIan Q Whishaw
Jun 12, 2020·Frontiers in Neuroscience·Justin ShenkAmanda J Kiliaan
Oct 14, 2020·Scientific Reports·Ning Cheng, Kartikeya Murari
Mar 14, 2021·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Erin E BrandtNatasha Mhatre
Jul 4, 2020·Journal of Neuroscience Methods·H KloefkornS Hochman
Apr 7, 2021·Nature Methods·Mathew L RynesSuhasa B Kodandaramaiah
May 4, 2021·Frontiers in Systems Neuroscience·Masashi KoizumiKazuhiko Seki
Aug 8, 2021·Animals : an Open Access Journal From MDPI·Elizabeth A NunamakerPatricia V Turner

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Methods Mentioned

BETA
transgenic

Software Mentioned

MATLAB
Picamera
Python
openCV
MATLAB Code
RaspiMJPEG
Apache
Icy
RaspberryPi
Chronux Toolbox

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