Low-density expansion protects human synovium-derived stem cells from replicative senescence: a preliminary study

Drug Delivery and Translational Research
Jingting LiMing Pei

Abstract

Our hypothesis in this study is that low seeding density expansion could retain human synovium-derived stem cell (hSDSC) "stemness", defined as higher proliferation and multi-differentiation capacity; retention of "stemness" probably occurs through the mitogen-activated protein kinase (MAPK) signaling pathway. hSDSCs were expanded in conventional plastic flasks for two consecutive passages at either low or high density (30 or 3,000 cells/cm(2)). Expanded cells were assessed for the effect of seeding density on their morphology, proliferation, apoptosis, stem cell surface markers, and multi-lineage differentiation capacity (chondrogenic, adipogenic, and osteogenic differentiation) using flow cytometry, biochemical analysis, histology, immunostaining, and real-time polymerase chain reaction. The MAPK signaling pathway (Erk1/2, p38, and JNK) and senescence-associated markers (p21 and caveolin) were also evaluated for their role in cell density-based monolayer expansion using western blot. Our data suggested that low seeding density expansion yielded hSDSCs with enhanced proliferation and multi-differentiation capacity compared to those grown at high seeding density, despite the fact that the cells expanded at both high and low den...Continue Reading

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