Low density lipoprotein aged in plasma forms clusters resembling subendothelial droplets: aggregation via surface sites

Biophysical Journal
Marco De SpiritoTiziana Parasassi

Abstract

In early phases of atherogenesis, droplets and vesicles accumulate in the subendothelial extracellular space of arterial intima. There is much evidence to suggest that these droplets, ranging between 100 and 400 nm, derive from modified low-density lipoprotein (LDL). In investigations of the formation mechanism of these droplets, LDL fusion was previously induced in vitro by proteolysis, lipolysis, oxidation, and vigorous shaking, but all treatments failed to reproduce the size distribution range of in vivo droplets, mostly resulting, instead, in particles with a diameter intermediate between that of one and two LDL. Our approach was meant to mimic LDL aging in plasma. LDL isolated from plasma that was incubated overnight at 37 degrees C is slightly modified in the secondary structure of its protein component and is primed to form very large aggregates according to a reaction-limited mechanism. This mechanism requires interactions between selected surface sites, whereas massive fusion is ruled out. In the frame of the general theory for colloids, the aggregation of LDL aged in plasma fulfills all the requirements of the reaction-limited mechanism, encompassing 1), exponential growth; 2), fractal structure, with the dimension of...Continue Reading

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Citations

Jul 1, 2010·European Biophysics Journal : EBJ·Massimiliano PapiAlessandro Arcovito
Jun 3, 2008·Biochimica Et Biophysica Acta·Kira A MarkossianBoris I Kurganov
Sep 14, 2016·World Journal of Gastroenterology : WJG·Gabriele CiascaMarco De Spirito
Sep 10, 2014·Biomolecular Concepts·Mengxiao Lu, Olga Gursky
Feb 23, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tiziana ParasassiFulvio Ursini
Nov 14, 2013·Chembiochem : a European Journal of Chemical Biology·Valentina PalmieriMarco De Spirito
Aug 1, 2013·Annals of Clinical Biochemistry·Toshihiro SakuraiHitoshi Chiba
Apr 21, 2007·Biochemistry·Shobini JayaramanOlga Gursky
Jul 31, 2010·Biochemistry·Roberto BrunelliTiziana Parasassi
Aug 16, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Gabriele CiascaAntonio Bianconi
Nov 1, 2006·Biochemistry·Kira A MarkossianBoris I Kurganov

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