PMID: 3214455Jul 1, 1988Paper

Low density lipoprotein binding affinity of arterial wall isomeric chondroitin sulfate proteoglycans

Atherosclerosis
S R SrinivasanG S Berenson

Abstract

Although the selective interaction of low density lipoproteins (LDL) with arterial proteoglycans is known, information is lacking on LDL-binding affinity of different subspecies occurring within a proteoglycan family. Isomeric chondroitin sulfate proteoglycan preparations sedimenting at densities of 1.54 g/ml (D1), 1.50 g/ml (D2) and 1.46 g/ml (D3) were isolated from bovine aorta intima-media under dissociative conditions and subjected to equilibrium binding to LDL-agarose gel. D1, D2 and D3 contained 36%, 37% and 11% dermatan sulfate, respectively. Sulfate to hexosamine ratio was low (0.73) in D1 when compared to D2 and D3 (0.94 and 1.04). Of the total proteoglycans contained in D1, D2 and D3, 41%, 52% and 66% interacted with LDL, respectively. LDL-bound proteoglycans dissociated over a wide range of ionic strengths (0.15-1.0); in comparison, LDL-bound heparin dissociated within a narrow range (0.5-0.75). Unlike other preparations, 30% of bound D3 dissociated at an ionic strength of 1.0. In D1 and D2 the proportion of dermatan sulfate increased in proteoglycan fractions that were bound firmly to LDL, whereas a high affinity fraction in D3 contained no dermatan sulfate. Thus, isomeric chondroitin sulfate proteoglycans display c...Continue Reading

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Citations

Apr 7, 1993·Biochimica Et Biophysica Acta·M GigliV Rizzo
Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·B RadhakrisnamurthyG S Berenson
Dec 1, 1990·Matrix : Collagen and Related Research·G M CherchiG De Luca
Aug 2, 2006·Macromolecular Bioscience·Sabrina Cattaruzza, Roberto Perris
Feb 28, 1990·Biochemical and Biophysical Research Communications·L J Hronowski, T P Anastassiades
Dec 7, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mario MazzucatoRoberto Perris

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