PMID: 7536711Apr 1, 1995Paper

Low-density lipoprotein induces vascular adhesion molecule expression on human endothelial cells

Hypertension
H HallerF C Luft

Abstract

We tested the hypothesis that low-density lipoprotein (LDL) and its acetylated form influence surface expression of vascular adhesion molecules on human endothelial cells. Vascular adhesion molecule surface expression was assessed with flow cytometry on cultured endothelial cells with a modified enzyme-linked immunosorbent assay. LDL acetylation was determined by chromatography. Monocyte adhesion to endothelial cells was assessed with U937 cells by direct counting. Tumor necrosis factor-alpha (10 ng/mL), a positive control, induced a time-dependent expression of vascular adhesion molecules (P < .05), which peaked at 5 hours. Incubation of endothelial cells with LDL (1.3 to 26.0 mmol/L) led to an increase in expression at 2 and 5 hours (P < .05). Prolonged (24-hour) exposure to LDL resulted in a second peak. The effect of acetylated LDL on expression was not different from that of native LDL. Incubation with the protein kinase C inhibitor staurosporine (5 x 10(-8) mol/L) blocked the effects of both native and acetylated LDL completely (P < .05). The calcium channel blocker nitrendipine (10(-7) mol/L) did not influence the expression of vascular adhesion molecule at 2 and 5 hours but did reduce the effect of LDL on expression at ...Continue Reading

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Citations

Feb 16, 1999·Biochimica Et Biophysica Acta·Y ZhuM B Stemerman
Dec 20, 1996·Atherosclerosis·J H LinM B Stemerman
Sep 18, 1998·Diabetes Research and Clinical Practice·H Haller
Sep 27, 2000·Journal of Diabetes and Its Complications·G RenierO Serri
Mar 8, 2003·Journal of Diabetes and Its Complications·Geneviève RenierOmar Serri
Sep 16, 2005·Endothelium : Journal of Endothelial Cell Research·Imre KovácsAlbert Császár
Aug 1, 1996·Kidney International·H HallerF C Luft
Apr 30, 2002·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Andreas AmbroschWolfgang König
Sep 20, 2006·Journal of Biomedical Materials Research. Part a·M MartinesiF Borgioli
May 14, 2003·Histochemistry and Cell Biology·Udo JeschkeRenate Stahn
Jul 17, 2013·Journal of Cellular and Molecular Medicine·Jae-Hyun LeeJung-Won Park
Apr 10, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S-H ChoS L Johnston
Feb 22, 2001·Journal of the American Society of Nephrology : JASN·Kathrin HeermeierJan Galle
Feb 29, 2000·Metabolism: Clinical and Experimental·G RenierO Serri

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