Low-dose 5-fluorouracil induces cell cycle G2 arrest and apoptosis in keloid fibroblasts

The British Journal of Dermatology
L HuangAndrew Burd

Abstract

Intralesional injection of low-dose 5-fluorouracil (5-FU) has recently been used as an experimental modality for treating keloid scarring and has shown promising efficacy in improving scar appearance and preventing recurrence of the keloid. We sought to explore the cellular- and molecular-based evidence for the observed clinical benefits. Primary cell lines of keloid fibroblasts were treated with 5-FU at a range of lower doses (∼10 mg mL(-1) ) in monolayer culture and subjected to examination for cell viability, proliferative potential, apoptosis, cell cycle and associated proteins involved in cell cycle control. 5-FU significantly inhibited cell proliferation of keloid fibroblasts in the full dose range used in this study. The DNA synthesis was completely inhibited by 5-FU at 72 h, and significant cell apoptosis was observed at concentrations ≥ 1 mg mL(-1) for a period over 72 h. 5-FU caused a significant delay in cell cycle progression and the G2/M phase arrest. 5-FU induced p53 and p21 accumulation together with a decrease in cyclin B1 and Bcl-2 levels in treated keloid fibroblasts. Our data indicate that low-dose 5-FU (as low as 1 mg mL(-1) ) induces significant inhibition of proliferation, G2/M cell cycle arrest and apopto...Continue Reading

References

Oct 20, 1998·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·D A LadinD P Fivenson
Jan 29, 2000·Journal of Dermatological Science·P TeofoliP Puddu
Jul 1, 2005·Plastic and Reconstructive Surgery·Neil W BulstrodeAlison D Cambrey
Jul 24, 2007·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·P Durani, A Bayat
Aug 2, 2008·Journal of Biomedical Materials Research. Part a·Lin HuangAndrew Burd
Oct 8, 2008·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Wei LiuNan Song

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Citations

Jul 3, 2013·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Lin HuangAndrew Burd
Dec 5, 2013·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Jennifer A LedonKeyvan Nouri
Jul 2, 2011·The American Journal of Chinese Medicine·Ya-Jun LinGang Hu
Aug 2, 2012·American Journal of Clinical Dermatology·Lauren L Levy, Joshua A Zeichner
Jul 16, 2014·Journal of Endourology·Ugur UyeturkMehmet Emin Ozyalvacli
Feb 11, 2015·International Journal of Dermatology·Rui ChenZhihong Fan
Aug 14, 2013·Expert Opinion on Pharmacotherapy·Chenyu Huang, Rei Ogawa
May 23, 2016·Annales de chirurgie plastique et esthétique·C JalouxC Philandrianos
Oct 10, 2018·Integrative Cancer Therapies·Jae-Woo ParkJinsung Kim
Oct 27, 2018·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Jill S WaibelAlexander Daoud
Jun 9, 2020·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Sebastian P NischwitzLars-Peter Kamolz
Mar 1, 2020·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Kriistiina E HietanenIlkka S Kaartinen
Dec 30, 2017·Experimental and Therapeutic Medicine·Hongyi WangKai Tao
May 1, 2018·Dermato-endocrinology·Chao ShiDegang Yang

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis