Low-dose automated electron tomography: a recent implementation

Journal of Structural Biology
B K RathJ Frank

Abstract

Low-dose automated tomography has been implemented on a 400-kV JEOL intermediate voltage electron microscope. Instrumentation and procedures for automatic tomographic series data collection are described. Difficulties encountered and ways to overcome them are discussed. A low-dose tomographic projection series of a triad junction of frog sartorius muscle was semiautomatically collected and a 3-D reconstruction of this organelle was made.

References

Oct 1, 1992·Ultramicroscopy·A J KosterD A Agard
Oct 1, 1995·Ultramicroscopy·P PenczekJ Frank
Jan 1, 1996·Journal of Structural Biology·M Marko, A Leith
Jan 1, 1997·Biophysical Journal·R GrimmW Baumeister

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Citations

Jun 29, 2000·Trends in Biochemical Sciences·T G Frey, C A Mannella
Aug 6, 2002·Journal of Structural Biology·Joachim FrankCarmen A Mannella
Aug 6, 2002·Journal of Structural Biology·Bruce F McEwenCarmen Mannella
Aug 6, 2002·Journal of Structural Biology·Chyong-Ere HsiehCarmen A Mannella
Feb 2, 2015·The Review of Scientific Instruments·F J Timmermans, C Otto
Jun 5, 2012·Micron : the International Research and Review Journal for Microscopy·Jose-Jesus Fernandez
Dec 16, 2006·Journal of Structural Biology·Shawn Q ZhengDavid A Agard
Jun 15, 2004·Journal of Structural Biology·Qingxiong S ZhengDavid A Agard
Sep 27, 2005·Journal of Structural Biology·David N Mastronarde
Nov 5, 2002·Biophysical Journal·T WagenknechtM Marko
Jun 28, 2011·Journal of Structural Biology·Andreas KorinekJürgen M Plitzko
Nov 26, 2008·Journal of Structural Biology·Christian RenkenCarmen A Mannella
May 18, 2005·Biochimica Et Biophysica Acta·Brad J Marsh
Jan 27, 1998·Journal of Structural Biology·Y LiJ Frank
Jan 27, 1998·Journal of Structural Biology·A J KosterW Baumeister
May 1, 1999·Journal of Structural Biology·M Hadida-HassanM H Ellisman
May 14, 2004·Annual Review of Biophysics and Biomolecular Structure·Sriram Subramaniam, Jacqueline L S Milne
Aug 1, 2006·Journal of Structural Biology·Anja SeybertAchilleas S Frangakis
Jul 13, 2016·Scientific Reports·Jinxin LiuGang Ren
Apr 17, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B F McEwen, M Marko

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