Low Dose Combined Treatment with Ultraviolet-C and Withaferin a Enhances Selective Killing of Oral Cancer Cells

Antioxidants
Sheng-Yao PengHsueh-Wei Chang

Abstract

Withaferin A (WFA), a Withania somnifera-derived triterpenoid, is an anticancer natural product. The anticancer effect of nonionizing radiation such as ultraviolet-C (UVC) as well as the combined treatment of UVC and WFA is rarely investigated. Low dose UVC and/or WFA treatments (12 J/m2 and/or 1 μM) were chosen to evaluate antioral cancer cell line effects by examining cytotoxicity, cell cycle disruption, apoptosis induction, and DNA damage. For two cancer cell lines (Ca9-22 and HSC-3), single treatment (UVC or WFA) showed about 80% viability, while a combined treatment of UVC/WFA showed about 40% viability. In contrast, there was noncytotoxicity to normal oral cell lines (HGF-1). Compared to single treatment and control, low dose UVC/WFA shows high inductions of apoptosis in terms of flow cytometric detections for subG1, annexin V, pancaspase changes as well as Western blotting for detecting cleaved poly (ADP-ribose) polymerase (c-PARP) and caspase 3 (c-Cas 3) and luciferase assay for detecting Cas 3/7 activity. Low dose UVC/WFA also showed high inductions of oxidative stress and DNA damage in terms of flow cytometric detections of reactive oxygen species (ROS), mitochondrial superoxide (MitoSOX) generation, and membrane pote...Continue Reading

References

May 27, 2003·Apoptosis : an International Journal on Programmed Cell Death·J D LyA Lawen
Mar 8, 2005·Mutation Research·Jean CadetThierry Douki
Apr 19, 2007·Cancer Metastasis Reviews·Benjamin J MoellerMark W Dewhirst
Aug 8, 2009·Photochemistry and Photobiology·Thomas P Coohill, Jose-Luis Sagripanti
Jun 8, 2011·The Journal of Biological Chemistry·Seiji AdachiOsamu Kozawa
Oct 4, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Eun Sun YangTaeg Kyu Kwon
Sep 13, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chi-Chen YehHsueh-Wei Chang
Feb 7, 2013·Medicina oral, patología oral y cirugía bucal·Shao-Hui Huang, Brian O'Sullivan
Feb 26, 2015·Nucleic Acids Research·Valentina Turinetto, Claudia Giachino
Sep 25, 2017·Frontiers in Physiology·Hsueh-Wei ChangChing-Yu Yen
Apr 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hurng-Wern HuangHsueh-Wei Chang
May 29, 2018·Journal of Biomolecular Techniques : JBT·Amos Bairoch
Jun 28, 2018·Journal of Clinical and Experimental Dentistry·Baharan-Ranjbar OmidiAilin Mahdkhah
Jul 4, 2018·Biochemical and Biophysical Research Communications·Shuxian XiaSide Liu
Nov 9, 2018·Frontiers in Pharmacology·Bee Ling TanHeshu Sulaiman Rahman
May 23, 2019·Environmental Toxicology·Jen-Yang TangHsueh-Wei Chang
Aug 25, 2019·International Journal of Cancer. Journal International Du Cancer·Thibault RobinAmos Bairoch

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
flow cytometry

Software Mentioned

Google scholar
JMP12
FlowJo

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis