Low-dose Exogenous Ouabain Alleviates Cardiac Lipotoxicity Through Suppressing Expression of CD36

Journal of Cardiovascular Pharmacology
Ning GuoXiaolin Xue

Abstract

CD36 is a key transporter involved in fatty acid (FA) uptake and contributes to the accumulation of FA in cardiomyocytes. The objective of this study was to investigate the role of ouabain, a glycoside regulator of Na(+)/K(+)-ATPase, in the regulation of CD36 expression and FA accumulation. FATP1 transgenic (Tg) mice with lipotoxic cardiomyopathy displayed significantly increased cardiac CD36 expression and free fatty acid accumulation. The data on enzyme-linked immunosorbent assay showed that endogenous ouabain was decreased in the serum of Tg mice versus wild-type mice. CD36 expression and free fatty acid accumulation in their primary cardiomyocytes were abated by treatment with 0.15-0.30 μM ouabain. CD36 expression was suppressed by 0.2 μM ouabain treatment, and the suppression was rescued by C-reactive protein. CD36 expression and free fatty acid accumulation in the heart were markedly reduced in Tg mice injected with 30 or 40 ng of ouabain (P < 0.01). Obvious fatty infiltration was found in noninjected Tg mice but not in the mice injected with 40 ng of ouabain. In conclusion, low-dose exogenous ouabain increased Na(+)/K(+)-ATPase activity, suppressed C-reactive protein-mediated CD36 expression, and alleviated murine cardia...Continue Reading

References

Apr 15, 2000·The Journal of Biological Chemistry·A L WooJ B Lingrel
May 25, 2002·European Journal of Biochemistry·Zijian Xie, Amir Askari
Dec 25, 2004·Circulation Research·Hsiu-Chiang ChiuJean E Schaffer
Jul 5, 2005·Journal of Ethnopharmacology·Geneviève Philippe, Luc Angenot
Oct 4, 2005·Biochimica Et Biophysica Acta·Debby P Y KoonenJoost J F P Luiken
Jun 1, 2006·Acta Physiologica·M I PanayiotidisJ A Cidlowski
Mar 9, 2010·Biochemical and Biophysical Research Communications·Peter KolkhofJan Torzewski
May 4, 2010·Journal of Molecular and Cellular Cardiology·Luciana SapiaMartin Vila Petroff
Dec 15, 2010·Diabetes Research and Clinical Practice·Arpita ChakrabortyMaitree Bhattacharyya
Aug 2, 2011·Cardiovascular Research·Tineke van de WeijerPatrick Schrauwen
Oct 27, 2012·Journal of Lipid Research·Andrew N CarleyE Douglas Lewandowski
Feb 26, 2014·Biochimica Et Biophysica Acta·Syed J KhundmiriEleanor D Lederer
Mar 1, 2014·International Journal of Medical Sciences·Alexandra ParadisLubo Zhang
Jul 10, 2014·Journal of Neurotrauma·Moran Dvela-LevittDavid Lichtstein
Dec 8, 2014·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Antonin LamaziereFrancesco Visioli
Feb 26, 2015·Pharmacological Reports : PR·Eduardo F MeiraAlessandra S Padilha

❮ Previous
Next ❯

Citations

Aug 28, 2020·Archives of Oral Biology·Raquel Cristine Silva BarcelosCarlos Alexandre de Souza Bier

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Cardiotoxicity

Cardiotoxicity refers to the muscle damage or dysfunction of heart electrophysiology caused by drug intake or due to disease complications. It is a well-known side effect of several cytotoxic drugs, especially of the anthracyclines and can lead to long term morbidity. Here is the latest research.

Cardiovascular Homeostasis

Cardiovascular regulation and homeostasis is important in maintaining health and dysfunction may lead to cardiovascular diseases. Nitric oxide and vascular endothelium are one of many molecules involved in the homeostatic mechanism. Here is the latest research on cardiovascular homeostasis.