Low dose inflammatory potential of silica particles in human-derived THP-1 macrophage cell culture studies - Mechanism and effects of particle size and iron

Chemico-biological Interactions
Gayatri PremshekharanValerie Jean Leppert

Abstract

Silica and iron are major constituents in ambient particulate matter, and iron is a common impurity in many engineered nanomaterials. The purpose of this work was to determine the pro-inflammatory and other biological effects and mechanism of particle size and iron presence under low dose, non-cytotoxic conditions that are likely to approximate actual exposure levels, in contrast with higher dose studies in which cytotoxicity occurs. Specifically, human-derived THP-1 macrophages were exposed to 1 μg/ml of pristine and iron-coated 50 nm and 2 μm engineered silica nanoparticles. Particles were first characterized for size, size distribution, surface area, iron concentration, phase and aggregation in cell culture media. Then, biological assays were conducted to determine a non-lethal dose used in subsequent experiments. Superoxide production, lipid peroxidation, and increased pro-inflammatory cytokine (TNF-α and IL-1β) mRNA expression were measured as a function of particle size and iron presence. Smaller particle size and the presence of iron increased superoxide production, lipid peroxidation, and the induction of pro-inflammatory cytokine mRNA expression. Separate addition of an iron-chelator, a scavenger of superoxide and hydr...Continue Reading

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Citations

Mar 23, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhouyang LiZhi-Hua Chen
Aug 29, 2018·Nanomedicine·Liangjiao ChenLongquan Shao
Feb 1, 2019·Environmental Science. Processes & Impacts·Ajith PattammattelPeggy A O'Day
Nov 28, 2020·International Journal of Nanomedicine·Xuemeng DongZhiwei Sun

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