Low dose of chronic ethanol exposure in adult zebrafish induces hepatic steatosis and injury

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Ki-Hoon Park, Seok-Hyung Kim

Abstract

Chronic alcohol consumption is a major cause of chronic liver disease worldwide. Adult zebrafish have emerged as a new vertebrate model of alcoholic liver disease. In previous research, a high dose of chronic ethanol treatment induced characteristic features of steatosis and hepatic injury in adult zebrafish, yet the ethanol concentration in that study was significantly higher than the lethal dose in humans. In the current study, we examined whether a low dose of chronic ethanol exposure in adult zebrafish induced the metabolic and pathological features seen in alcoholic liver disease. We found that chronic ethanol treatment at 0.2% ethanol (v/v) concentration for 4 weeks induced a significant elevation of serum glucose and triacylglycerol in adult zebrafish. In addition, serum alanine aminotransferase activity was significantly elevated after ethanol treatment. Histological analysis revealed steatosis and hepatocyte ballooning phenotype. Gene expression analysis using quantitative real-time PCR suggested that ethanol treatment induced inflammation, apoptosis, and fibrosis. In addition, we found significant increases in gene expression involved in glucose and lipid metabolism as well as mitochondrial biogenesis and function. Im...Continue Reading

Citations

Jul 14, 2020·Journal of Applied Toxicology : JAT·Swati Katoch, Vikram Patial
Dec 24, 2019·Frontiers in Immunology·Arkadi ShwartzChunyue Yin
Jan 31, 2021·The Journal of Pharmacology and Experimental Therapeutics·Leilei ZhangKenneth D Tew
Mar 30, 2021·Journal of Aquatic Animal Health·Mine KöktürkMuhammed Atamanalp
Jul 3, 2021·International Journal of Molecular Sciences·Xixin WangPeter A M de Witte
Sep 10, 2021·Laboratory animal research·Chong Pyo ChoeHyunju Ro

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