Abstract
Hormesis and adaptive responses are 2 important biological effects of low-dose ionizing radiation (LDR). In normal tissue, LDR induces hormesis as evinced by increased cell proliferation; however, whether LDR also increases tumor cell proliferation needs to be investigated. In this study, cell proliferation was assayed by total cell numbers and the Cell Counting Kit 8 assay. Mitogen-activated protein kinases (MAPK)/extracellular signal-regulated kinase (ERK) and phosphatidylinositol 3' -kinase(PI3K)-Akt (PI3K/AKT) phosphorylation were determined by Western blot analysis. Human embryonic lung fibroblast 2BS and lung cancer NCI-H446 cell lines were irradiated with LDR at different doses (20-100 mGy). In response to 20 to 75 mGy X-rays, cell proliferation was significantly increased in 2BS but not in NCI-H446 cells. In 2BS cells, LDR at 20 to 75 mGy also stimulated phosphorylation of MAPK/ERK pathway proteins including ERK, MEK, and Raf and of the PI3K/AKT pathway protein AKT. To test whether ERK1/2 and AKT pathway activation was involved in the stimulation of cell proliferation in 2BS cells, the MAPK/ERK and PI3K/AKT pathways were inhibited using their specific inhibitors, U0126 and LY294002. U0126 decreased the phosphorylation o...Continue Reading
References
Apr 21, 1995·Mechanisms of Ageing and Development·J LiT Tong
Oct 20, 2001·Mechanisms of Ageing and Development·X ZhuM A Smith
Oct 25, 2001·Proceedings of the National Academy of Sciences of the United States of America·N N KhodarevR R Weichselbaum
Feb 26, 2004·Radiation Research·B MarplesM C Joiner
Jul 9, 2004·Biochemical and Biophysical Research Communications·Nitaya IndrawattanaAhnond Bunyaratvej
Nov 13, 2004·Experimental Hematology·Wei LiLu Cai
Apr 28, 2005·Oncogene·Kathryn MercerCatrin Pritchard
Nov 28, 2006·Biochimica Et Biophysica Acta·James A McCubreyRichard A Franklin
Jul 31, 2007·Journal of Radiation Research·Cha Soon KimJoon Kim
Feb 26, 2008·Journal of Radiation Research·Hongyu JiangGuanjun Wang
Feb 13, 2009·Biochimica Et Biophysica Acta·Reut KornasioOrna Halevy
Oct 21, 2010·International Journal of Radiation Biology·Mengde CaoDavid Nelson
Mar 26, 2011·Journal of Radiation Research·Xinyue LiangWei Li
Oct 4, 2011·BMC Cancer·Christoph ReissfelderJürgen Weitz
Sep 4, 2012·Oral Oncology·Naseem GhazaliJanet M Risk
Sep 14, 2012·The British Journal of Radiology·D GuiradoA M Lallena
Feb 27, 2013·Cancer·Jiemin MaAhmedin Jemal
Sep 3, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Hong-Sheng YuHao Wang
Dec 7, 2013·The Lancet Oncology·Gregory P Kalemkerian
Dec 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Hiromichi EbiJeffrey A Engelman
Jan 1, 2014·Plastic and Reconstructive Surgery·Justin R HubenakSteven J Kronowitz
Jan 25, 2014·Nature Reviews. Cancer·Nicola McCarthy
Feb 4, 2014·Journal of Geriatric Oncology·Maryska L G Janssen-HeijnenA N Machteld Wymenga
Nov 18, 2014·Cancer Biotherapy & Radiopharmaceuticals·Guozi YangJiuwei Cui
Nov 22, 2014·Biological Trace Element Research·Takashi HimotoTsutomu Masaki
Dec 3, 2014·Clinical Lung Cancer·Steven Lehrer, Kenneth E Rosenzweig
Citations
Jun 15, 2016·International Journal of Cancer. Journal International Du Cancer·Guozi YangJiuwei Cui
Jan 31, 2017·International Journal of Molecular Sciences·Jiuwei CuiLu Cai
Jan 21, 2017·Archives of Biochemistry and Biophysics·Heba H MansourShereen M Galal
Sep 8, 2017·International Journal of Radiation Biology·Hyeon Soo EomSeon Young Nam
Jun 29, 2018·International Journal of Radiation Biology·Ilya O VelegzhaninovDmitry Yu Klokov
Jan 5, 2018·PloS One·Katelyn TruongDelphine Dean
Jan 24, 2019·Biology Open·Soojin HwangSeon Young Nam
Jan 25, 2019·International Journal of Radiation Biology·Kavitha PremkumarBhavani S Shankar
Jul 18, 2018·Acta Microbiologica Et Immunologica Hungarica·György Csaba
Nov 23, 2017·Aging·Margarita PustovalovaAndreyan N Osipov
Mar 6, 2019·Dose-response : a Publication of International Hormesis Society·Kaihua JiQiang Liu
Apr 9, 2020·The Review of Scientific Instruments·Bishwambhar SenguptaEndre Takacs
May 20, 2020·Dose-response : a Publication of International Hormesis Society·Clément DevicNicolas Foray
Mar 13, 2019·Dose-response : a Publication of International Hormesis Society·Cuihua LiuBing Wang
Dec 8, 2019·International Journal of Molecular Sciences·Annemarie SchröderKatrin Manda
Jun 17, 2020·Molecular Biology Reports·Shereen M El KikiHesham F Hasan
Jun 1, 2017·Oncology Reports·Yang WangDexin Yin
Aug 8, 2020·Medical Hypotheses·Vladimir M Subbotin
Sep 13, 2019·Ecotoxicology and Environmental Safety·Jieran AnShumao Ding
Nov 3, 2020·Frontiers in Genetics·Eunguk ShinBuHyun Youn
Feb 26, 2021·Dose-response : a Publication of International Hormesis Society·Bing WangMitsuru Nenoi
Jul 25, 2021·International Journal of Molecular Sciences·Abrar Ul Haq KhanYevgeniya Le
Aug 9, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Edward J Calabrese