Low-dose radiations derived from cone-beam CT induce transient DNA damage and persistent inflammatory reactions in stem cells from deciduous teeth

Dento Maxillo Facial Radiology
Piroska ViragReinhilde Jacobs

Abstract

Cone-beam CT (CBCT), a radiographic tool for diagnosis, treatment, and follow-up in dental practice, was introduced also in pediatric radiology, especially orthodontics. Such patients subjected to repetitive X-rays examinations may receive substantial levels of radiation doses. Ionizing radiation (IR), a recognized carcinogenic factor causing DNA double-strand breaks (DSBs) could be harmful to undifferentiated cells such as dental pulp stem cells (DPSCs) since inaccurately repaired or unrepaired DSBs may lead to malignant transformation. The H2AX and MRE11 proteins generated following DSBs formation and pro-inflammatory cytokines (CKs) secreted after irradiation are relevant candidates to monitor the cellular responses induced by CBCT. DPSCs were extracted from human exfoliated deciduous teeth and their phenotype was assessed by immunocytochemistry and flow-cytometry. Cells were exposed to IR doses: 5.4-107.7 mGy, corresponding to 0.5-8 consecutive skull exposures, respectively. H2AX and MRE11 were detected in whole cells, while IL-1α, IL-6, IL-8, TNFα in supernatants, using enzyme-linked immunosorbent assay (ELISA) at different time points after exposure. The phosphorylation level of H2AX in DPSCs increased considerably at 0.5...Continue Reading

References

Oct 25, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S T Ahmed, L B Ivashkiv
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·S GronthosS Shi
Apr 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kai Rothkamm, Markus Löbrich
Apr 1, 2004·British Journal of Cancer·H WillersS N Powell
Jun 30, 2004·Radiation Research·William H McBrideYu-Pei Liao
Jun 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Markus LöbrichMichael Uder
Jun 28, 2005·The FEBS Journal·Elinor R Foster, Jessica A Downs
Nov 17, 2009·The Journal of Biological Chemistry·Jingsong Yuan, Junjie Chen
Apr 30, 2011·The Journal of Biological Chemistry·Jamunarani VeeraraghavanNatarajan Aravindan
Dec 14, 2011·The Angle Orthodontist·Diego Coelho LorenzoniEduardo Franzotti Sant' Anna
Feb 7, 2012·The British Journal of Radiology·C TheodorakouUNKNOWN SEDENTEXCT Project Consortium
May 2, 2012·International Journal of Dentistry·Diego Coelho LorenzoniEduardo Franzotti Sant'anna
Jun 8, 2012·Frontiers in Oncology·Gabriele Multhoff, Jürgen Radons
Jun 9, 2012·Mutation Research·Eileen PernotElisabeth Cardis
Oct 31, 2012·Radiation Research·Dörthe SchaueWilliam H McBride
Jun 25, 2014·Physics in Medicine and Biology·Ruben PauwelsKeith Horner
Feb 24, 2015·Journal of Inflammation·Federica Maria Di MaggioValentina Bravatà
May 4, 2015·Radiation and Environmental Biophysics·Matthew L JohnstonKenneth L Shepard
Jan 23, 2016·Scientific Reports·Mari KatsuraHideko Sone
Mar 28, 2017·Mutation Research. Reviews in Mutation Research·Janet HallElisabeth Cardis

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Citations

May 11, 2021·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Iwona Gregorczyk-MagaPaweł Maga

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