Low Expression of Mfn2 Is Associated with Mitochondrial Damage and Apoptosis of Ovarian Tissues in the Premature Ovarian Failure Model

PloS One
Wenqi ChenWenpei Xiang

Abstract

This study aimed to construct a working model for detecting the mitochondrial damage and expression of Mfn2. It furthermore explored the pathogenesis of premature ovarian failure (POF) induced by cisplatin. Forty young female mice were divided randomly into two groups. The first was the treatment group intraperitoneally administered cisplatin (1.5mg/kg). The untreated control group was likewise injected with physiological saline for 10 days. One month later, we observed the ovarian weight and morphological changes, particularly the development of follicles and concentration of sex hormones. Immunohistochemistry and western blotting were used to measure the two groups. We later evaluated ovarian cell apoptosis with TUNEL and analyzed Bcl-2 and Bax levels. We used transmission electron microscopy in order to observe the ultrastructure of ovarian cells. The phosphomolybdic acid colorimetric method was used to measure the ATP content in the ovarian tissue. Finally, the mitochondrial membrane potential of ovarian cells was detected with JC-1 dye. The cisplatin resulted in a decline of body weight, reduced ovarian weight significantly, and resulted in disorders of the extrous cycle. The follicles' number decreased within the tissue's...Continue Reading

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Citations

Jan 24, 2018·Mediators of Inflammation·Lan YingZhong-Qiu Lu
Jan 18, 2019·Journal of the Endocrine Society·S A Masudul HoqueMasayuki Shimada
May 18, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Muladili YuemaierXiamixinuer Yilike
Sep 19, 2020·Endocrinology·Manasi DasNicholas J G Webster
Dec 15, 2016·Journal of Agricultural and Food Chemistry·Haili WuZhuoyu Li

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Methods Mentioned

BETA
GTPase
enzyme-linked immunosorbent assay
ELISA
PCR
electrophoresis
fluorescence
Assay
transmission electron microscopy
GTPases

Software Mentioned

pro plus
Quantity One
Statistical Program for Social Science ( SPSS
Image

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