Low-fat diet and exercise preserve eNOS regulation and endothelial function in the penis of early atherosclerotic pigs: a molecular analysis

The Journal of Sexual Medicine
Biljana MusickiArthur L Burnett

Abstract

Diet and exercise affect endothelial function in the penis, but the molecular mechanisms underlying their effects are not understood. We evaluated endothelial nitric oxide synthase (eNOS) interaction with its negative regulator caveolin-1 and eNOS uncoupling as molecular targets in the penis associated with the beneficial effects of low-fat diet and chronic exercise. The penes were obtained from adult male Yucatan pigs fed a normal-fat or high-fat diet on exercised or sedentary regimen for 24 weeks. Markers of endothelial function (guanosine 3',5'-monophosphate [cGMP] production), endothelial dysfunction (eNOS uncoupling and eNOS interaction with caveolin-1), and oxidative stress (thiobarbituric acid reactive substances [TBARS]) were measured in the penes. The concentrations of cGMP and TBARS were determined using commercial kits. eNOS uncoupling was determined by low-temperature sodium dodecyl sulfate polyacrylamide gel electrophoresis. eNOS binding to caveolin-1, eNOS phosphorylation (Ser-1177), and protein expression of eNOS and caveolin-1 were measured by Western blot analysis in penes purified for NOS and in homogenates, respectively. Molecular parameters of endothelial function including eNOS regulatory function. Relative...Continue Reading

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Citations

Sep 27, 2012·Journal of Physiology and Biochemistry·François GuerreroJacques Mansourati
Feb 4, 2011·International Journal of Impotence Research·J M JohnsonB Musicki
Jun 7, 2008·American Journal of Respiratory and Critical Care Medicine·Galia K Soukhova-O'HareDavid Gozal
Jan 28, 2009·The Journal of Sexual Medicine·Fernando S CarneiroR Clinton Webb
May 3, 2012·The Journal of Sexual Medicine·Olav SchjørringUlf Simonsen
Sep 9, 2009·The Journal of Sexual Medicine·Sue W Goldstein
Mar 18, 2010·The Journal of Sexual Medicine·Biljana MusickiArthur L Burnett
May 25, 2012·The Journal of Sexual Medicine·Biljana MusickiArthur L Burnett
Dec 15, 2010·The Journal of Sexual Medicine·Biljana MusickiArthur L Burnett
Jan 30, 2009·The Journal of Sexual Medicine·Shiu-Dong ChungJu-Ton Hsieh
May 22, 2010·The Journal of Sexual Medicine·Katherine EspositoDario Giugliano
May 22, 2010·The Journal of Sexual Medicine·Jae Hyun BaeDu Geon Moon
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Jun 16, 2009·The Journal of Sexual Medicine·Carla Costa, Ronald Virag
Sep 1, 2020·American Journal of Hypertension·Amanda Almeida de Oliveira, Kenia Pedrosa Nunes
Jun 14, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Justin D La FavorChristopher J Wingard

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