Low Fouling Protein Detection in Complex Biological Media Supported by a Designed Multifunctional Peptide

ACS Sensors
Nianzu LiuXiliang Luo

Abstract

The construction of sensitive and selective biosensors capable of detecting specific targets in complex biological samples remains a challenge highly relevant to a range of sensor/diagnostic applications. Herein, we have utilized a multifunctional peptide to present an interface that supports the very specific recruitment of targets from serum. The novel peptide sequence designed contains an anchoring domain (CPPPP-), an antifouling domain (-NQNQNQNQDHWRGWVA), and a human immunoglobulin G (IgG) recognition domain (-HWRGWVA), and the whole peptide was designed to be antifouling. These were integrated into polyaniline nanowire arrays in supporting the quantification of IgG (with a limit of detection of 0.26 ng mL-1) in neat serum and real clinical samples. The strategy of utilizing multisegment peptide films to underpin highly selective target recruitment is, of course, readily extended to a broad range of targets for which an affinity sequence can be generated.

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Citations

Sep 22, 2018·Chemical Communications : Chem Comm·Haishan QiLei Zhang
Jan 15, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jialian DingBin Su
Jan 10, 2020·The Analyst·Pei-Heng Lin, Bor-Ran Li
Apr 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nan LiFuquan Dang
Sep 12, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wenshi LiXiliang Luo
Mar 18, 2021·Acta Biomaterialia·Qi ChenRunhui Liu
Jun 17, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Lidya AbuneYong Wang
Mar 24, 2020·Chemical Reviews·Cheng JiangJason J Davis
Aug 27, 2021·Biosensors·Patrick Severin SfraganoIlaria Palchetti

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