PMID: 7539340May 1, 1995Paper

Low frequency of alterations in p53, K-ras, and mdm2 in rat lung neoplasms induced by diesel exhaust or carbon black

Carcinogenesis
D S SwaffordS A Belinsky

Abstract

Inhalation of diesel exhaust (DE), which contains soot particles with adsorbed mutagenic organic compounds, and its virtually mutagen-free soot particle analog, carbon black (CB), produce similar types and prevalences of pulmonary neoplasms in chronically exposed F344 rats. This result suggests that DE-induced neoplasia develops from the effects of a high lung burden of carbonaceous particles rather than from the genotoxicity of organic constituents. In this investigation, pulmonary carcinomas from rats exposed to DE or CB were analyzed for alterations in K-ras and p53 to determine if mutations caused by these agents are also similar. K-ras and p53 were chosen for this study because mutation patterns of these genes in lung neoplasms have been associated with specific exposures. A low frequency (3/50) and variable pattern of activating mutations were identified in codons 12 and 61 of the K-ras gene. Immunoreactive levels of p53 protein, suggesting gene dysfunction, were present in 7/13 squamous cell or adenosquamous carcinomas, regardless of the associated exposure. However, single-strand conformational polymorphism analysis and direct sequencing of p53 did not detect any mutations in these neoplasms. No immunoreactivity or muta...Continue Reading

Citations

Sep 4, 1995·Cancer Letters·N ShivapurkarO Alabaster
Jun 1, 1997·Environmental Health Perspectives·S A BelinskyK J Nikula
Feb 10, 1998·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·K IwaiY Kawabata
Mar 18, 2004·International Journal of Cancer. Journal International Du Cancer·Ad M KnaapenRoel P F Schins
Jul 22, 2004·Regulatory Toxicology and Pharmacology : RTP·L J RauschA Sharma
Mar 19, 1999·Japanese Journal of Cancer Research : Gann·M HouM Noguchi
Aug 27, 2005·Carcinogenesis·Paolo Vineis, Kirsti Husgafvel-Pursiainen
Feb 23, 2020·Archives of Toxicology·Wenting ChengShuguang Leng
May 11, 2004·Journal of Radiation Research·Yutaka YamadaGeorges Monchaux
Nov 3, 2017·Critical Reviews in Toxicology·Ishrat ChaudhuriLen Levy
Aug 21, 2020·Toxicological Sciences : an Official Journal of the Society of Toxicology·Xue CaoShuguang Leng
Mar 1, 1997·Molecular and Cellular Biology·D S SwaffordS A Belinsky

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