Abstract
Low-frequency vibration accelerates cartilage degeneration in knee osteoarthritis (KOA) rat model. In this article, we investigated whether whole-body vibration (WBV) increases cartilage degeneration by regulating tumor necrosis factor-α (TNF-α) in KOA. Proteomics analysis was used to filter candidate protein from synovial fluid (SF) in KOA people after WBV. Enzyme-linked immunosorbent assay (ELISA) was used to estimate changes in TNF-α levels in SF. The C57 mice and TNF-α knock-out mice were sacrificed for the KOA model and WBV intervention. The cartilage was tested by ELISA, histology, terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL), immunohistochemistry, and reverse transcriptase polymerase chain reaction. Luciferase activity test in vitro study was conducted to confirm the relationship between TNF-α and the candidate protein. Differentially expressed proteins were enriched in the glycolytic process, glucose catabolic, and regulation of interleukin-8 (IL-8) secretion processes. Phosphoglycerate kinase, triosephosphate isomerase 1, T cell immunoglobulin- and mucin-domain-containing molecules 2, fumarylacetoacetate hydrolase (FAH), and TNF were the hub node. TNF-α expression increased in SF after WBV (...Continue Reading
References
Apr 11, 1990·Nucleic Acids Research·E AgsteribbeR Berger
Aug 1, 1986·Arthritis and Rheumatism·R AltmanM Hochberg
May 1, 1996·The Journal of Clinical Investigation·P ReboulJ Martel-Pelletier
Jan 11, 2005·Arthritis and Rheumatism·Masahiko KobayashiA Robin Poole
Jan 16, 2007·Annals of the Rheumatic Diseases·Elke KunischRaimund W Kinne
Jun 20, 2007·The Journal of Cell Biology·Anna SpagnoliHarold L Moses
Sep 8, 2010·Osteoarthritis and Cartilage·O StannusC Ding
Oct 1, 2010·Osteoarthritis and Cartilage·S S GlassonC B Little
Dec 2, 2010·Nature Reviews. Rheumatology·Mohit KapoorHassan Fahmi
Mar 15, 2011·Joint, Bone, Spine : Revue Du Rhumatisme·Hua GuoChengqi He
Jun 18, 2011·Proteomics·Wei-Juan MaMikko J Lammi
Oct 12, 2011·Arthritis and Rheumatism·Hanna OppenheimerMona Dvir-Ginzberg
Sep 7, 2013·Osteoarthritis and Cartilage·Y LiA J Grodzinsky
May 24, 2014·Osteoarthritis and Cartilage·J QinW-H Cheung
Dec 20, 2014·Clinical Rehabilitation·Pu WangChengqi He
Apr 22, 2015·Arthritis & Rheumatology·Matthew R McCannCheryle A Séguin
May 9, 2015·Osteoarthritis and Cartilage·G K FitzgeraldK L Bennell
Oct 3, 2015·Clinical Rehabilitation·Pu WangChengqi He
Oct 26, 2016·Journal of Pineal Research·Azam HosseinzadehSaeed Mehrzadi
Oct 5, 2016·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Himalaya ParajuliDaniela Elena Costea
Jan 7, 2017·Journal of Cellular Physiology·Xiliang DuXinwei Li
Jan 21, 2017·Biochimie·Johannes F WentzelPiet J Pretorius
Jan 21, 2017·Osteoarthritis and Cartilage·Matthew R McCannCheryle A Séguin
Feb 23, 2017·Osteoarthritis and Cartilage·A G H MierzwaR D Reginato