Low-Frequency Vibration Promotes Tumor Necrosis Factor-α Production to Increase Cartilage Degeneration in Knee Osteoarthritis.

Cartilage
Peng-Ming YuCheng-Qi He

Abstract

Low-frequency vibration accelerates cartilage degeneration in knee osteoarthritis (KOA) rat model. In this article, we investigated whether whole-body vibration (WBV) increases cartilage degeneration by regulating tumor necrosis factor-α (TNF-α) in KOA. Proteomics analysis was used to filter candidate protein from synovial fluid (SF) in KOA people after WBV. Enzyme-linked immunosorbent assay (ELISA) was used to estimate changes in TNF-α levels in SF. The C57 mice and TNF-α knock-out mice were sacrificed for the KOA model and WBV intervention. The cartilage was tested by ELISA, histology, terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL), immunohistochemistry, and reverse transcriptase polymerase chain reaction. Luciferase activity test in vitro study was conducted to confirm the relationship between TNF-α and the candidate protein. Differentially expressed proteins were enriched in the glycolytic process, glucose catabolic, and regulation of interleukin-8 (IL-8) secretion processes. Phosphoglycerate kinase, triosephosphate isomerase 1, T cell immunoglobulin- and mucin-domain-containing molecules 2, fumarylacetoacetate hydrolase (FAH), and TNF were the hub node. TNF-α expression increased in SF after WBV (...Continue Reading

References

Apr 11, 1990·Nucleic Acids Research·E AgsteribbeR Berger
Jun 20, 2007·The Journal of Cell Biology·Anna SpagnoliHarold L Moses
Dec 2, 2010·Nature Reviews. Rheumatology·Mohit KapoorHassan Fahmi
Oct 12, 2011·Arthritis and Rheumatism·Hanna OppenheimerMona Dvir-Ginzberg
Oct 26, 2016·Journal of Pineal Research·Azam HosseinzadehSaeed Mehrzadi
Oct 5, 2016·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Himalaya ParajuliDaniela Elena Costea
Jan 7, 2017·Journal of Cellular Physiology·Xiliang DuXinwei Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis