Low-Intensity Ultrasound Modulates Ca2+ Dynamics in Human Mesenchymal Stem Cells via Connexin 43 Hemichannel

Annals of Biomedical Engineering
Chi Woo YoonK Kirk Shung

Abstract

In recent years, ultrasound has gained attention in new biological applications due to its ability to induce specific biological responses at the cellular level. Although the biophysical mechanisms underlying the interaction between ultrasound and cells are not fully understood, many agree on a pivotal role of Ca2+ signaling through mechanotransduction pathways. Because Ca2+ regulates a vast range of downstream cellular processes, a better understanding of how ultrasound influences Ca2+ signaling could lead to new applications for ultrasound. In this study, we investigated the mechanism of ultrasound-induced Ca2+ mobilization in human mesenchymal stem cells using 47 MHz focused ultrasound to stimulate single cells at low intensities (~ 110 mW/cm2). We found that ultrasound exposure triggers opening of connexin 43 hemichannels on the plasma membrane, causing release of ATP into the extracellular space. That ATP then binds to G-protein-coupled P2Y1 purinergic receptors on the membrane, in turn activating phospholipase C, which evokes production of inositol trisphosphate and release of Ca2+ from intracellular stores.

References

Jan 1, 1994·Molecular and Cellular Endocrinology·M J SandersonE R Dirksen
Sep 30, 1993·Nature·M J Berridge
Apr 24, 1997·Nature·M J Berridge
Aug 16, 2000·Cell Calcium·L MissiaenH De Smedt
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S Etienne-MannevilleP O Couraud
Feb 21, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Javad ParviziMark E Bolander
Jul 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gregory ArcuinoMaiken Nedergaard
Oct 12, 2004·American Journal of Physiology. Cell Physiology·Li BaoGerhard Dahl
Jan 10, 2006·Developmental Cell·A Wayne OrrMartin A Schwartz
Jun 6, 2006·Critical Reviews in Biomedical Engineering·Eitan Kimmel
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Jean Xin JiangSirisha Burra
Apr 25, 2008·Nature Reviews. Cancer·H Llewelyn Roderick, Simon J Cook
Nov 18, 2008·Ultrasound in Medicine & Biology·R E KumonC X Deng
Jan 27, 2009·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·Thomas R NelsonCharles C Church
Oct 13, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Z FanC X Deng
Feb 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Boris KrasovitskiEitan Kimmel
Feb 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Nidhi BatraJean X Jiang
Jul 17, 2012·The Journal of Membrane Biology·Mia M ThiDavid C Spray
Jul 20, 2012·Physiological Reviews·Luc Leybaert, Michael J Sanderson
Oct 9, 2012·Biotechnology and Bioengineering·Kwok Ho LamKirk Koping Shung
Mar 23, 2013·Frontiers in Molecular Neuroscience·Mercè Izquierdo-SerraPau Gorostiza
Jul 28, 2013·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·Kyung Shin KangDong-Woo Cho
Jul 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Guillermina M GoñiDaniel Lietha
Sep 22, 2016·Stem Cell Research & Therapy·E XiaoYi Zhang

❮ Previous
Next ❯

Software Mentioned

imageJ
KLM
MATLAB
GraphPad Prism
GraphPad

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.