PMID: 15253040Jul 16, 2004Paper

Low-level atrazine exposure decreases cell proliferation in human fibroblasts

Archives of Environmental Contamination and Toxicology
Michelle K ManskeKavita R Dhanwada

Abstract

There is continuing concern that release of certain chemicals into the environment leads to human exposure to toxins, particularly through contaminated ground and surface waters. The triazine herbicide atrazine (2-chloro-4-ethylamine-6-isopropylamino-S-triazine) is routinely found as a contaminant in many surface and groundwaters. Little is known of the long-term effects of atrazine exposure on human cells. The objective of this research was to examine whether low-level exposure of atrazine to normal human fibroblast cells in culture had measurable deleterious effects. We show that low-level, short-term exposure of atrazine to normal fibroblasts results in decreased cell proliferation. In two separate assays to measure cell proliferation, either a 1.5-fold decrease in the cell number occurred (saturation density analysis) or a 2-fold decrease in cell proliferation was found (MTT analysis). Experiments to measure apoptosis in cells exposed to low levels of atrazine did not show DNA fragmentation or increased caspase activity. Thus, this analysis shows that short-term exposure to environmentally relevant, low levels of atrazine can be assessed by a decrease in cellular proliferation of normal human cells.

Citations

Feb 22, 2008·Environmental Health Perspectives·Jenny R LenkowskiKelly A McLaughlin
May 10, 2008·Bulletin of Environmental Contamination and Toxicology·I KmeticJ Kniewald
Sep 17, 2009·Current Microbiology·William J Hunter, Dale L Shaner
Feb 22, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S O AbarikwuA B Pant
Oct 14, 2008·Journal of Contaminant Hydrology·William J Hunter, Dale L Shaner
Mar 11, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Davor ZeljezicPetra Perkovic
Jun 23, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S O AbarikwuA B Pant
Jul 6, 2011·Free Radical Research·Sunny O AbarikwuAditya B Pant
Nov 12, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ludovic PeyreRoger Rahmani
Jan 15, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Erin R PowellKavita R Dhanwada
Mar 10, 2015·Environmental Toxicology and Pharmacology·Huigang Liu, Juan Li
Mar 11, 2015·Pesticide Biochemistry and Physiology·Sunny O Abarikwu, Ebenezer O Farombi

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