Low-level Gestational Lead Exposure Alters Dendritic Spine Plasticity in the Hippocampus and Reduces Learning and Memory in Rats

Scientific Reports
Zai-Hua ZhaoJing-Yuan Chen

Abstract

Lead (Pb) is known to impair children's cognitive function. It has been previously shown that developmental Pb exposure alters dendritic spine formation in hippocampal pyramidal neurons. However, the underlying mechanism has not yet been defined. In this study, a low-level gestational Pb exposure (GLE) rat model was employed to investigate the impact of Pb on the spine density of the hippocampal pyramidal neurons and its regulatory mechanism. Pb exposure resulted in impaired performance of the rats in the Morris water maze tasks, and in decreased EPSC amplitudes in hippocampal CA3-CA1 regions. With a 3D reconstruction by the Imaris software, the results from Golgi staining showed that the spine density in the CA1 region was reduced in the Pb-exposed rats in a dose-dependent manner. Decreased spine density was also observed in cultured hippocampal neurons following the Pb treatment. Furthermore, the expression level of NLGN1, a postsynaptic protein that mediates synaptogenesis, was significantly decreased following the Pb exposure both in vivo and in vitro. Up-regulation of NLGN1 in cultured primary neurons partially attenuated the impact of Pb on the spine density. Taken together, our resultssuggest that Pb exposure alters spin...Continue Reading

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Citations

Jan 10, 2019·International Journal of Molecular Sciences·Alexis HoweAntiño R Allen
Feb 20, 2019·International Journal of Molecular Sciences·Sudip BanerjeeSnehalata A Pawar
Apr 17, 2020·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Viviane Altermann TorreRachel Krolow
Nov 25, 2020·Neural Regeneration Research·Jian-Zhu BoYan-Shu Zhang
Feb 13, 2021·Journal of Environmental Science and Health. Part C, Toxicology and Carcinogenesis·Gaochun ZhuHongping Chen
Apr 9, 2021·Biological Trace Element Research·Hasan Ul BannaZahangir Alam Saud
Mar 16, 2021·Frontiers in Cell and Developmental Biology·Zai-Hua ZhaoXue-Feng Shen
Mar 11, 2020·Ecotoxicology and Environmental Safety·Dannilo Roberto Ferreira da SilvaRafael Rodrigues Lima

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Methods Mentioned

BETA
PCR
dissection

Software Mentioned

Imaris Bitplane
BSW
DP
Imaris
ImageJ
Statistical Package for the Social Sciences ( SPSS

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