Low-level laser therapy induces the expressions of BMP-2, osteocalcin, and TGF-β1 in hypoxic-cultured human osteoblasts.

Lasers in Medical Science
Se-Jeong PyoYong-Deok Kim

Abstract

The aim of this study was to examine the effect of low-level laser therapy (LLLT) on the cell viability and the expression of hypoxia-inducible factor-1s (HIF-1s), bone morphogenic protein-2 (BMP-2), osteocalcin, type I collagen, transforming growth factor-β1 (TGF-β1), and Akt in hypoxic-cultured human osteoblasts. Human fetal osteoblast cells (cell line 1.19) were cultured under 1 % oxygen tension for 72 h. Cell cultures were divided into two groups. At the experimental side, low-level laser (808 nm, GaAlAs diode) was applied at 0, 24, and 48 h. After irradiation, each cell culture was incubated 24 h more under hypoxia. Total energy was 1.2, 2.4, and 3.6 J/cm(2), respectively. Non-irradiated cultures served as controls. Comparisons between the two groups were analyzed by t test; a p value <0.05 was considered statistically significant. Hypoxia resulted in a decrease in the expression of type I collagen, osteocalcin, and TGF-β1 (p < 0.001, p < 0.001, and p < 0.01, respectively). Cell viability and BMP-2 expression were not decreased by hypoxic condition. On the other hand, LLLT on hypoxic-cultured osteoblast promoted the expression of BMP-2, osteocalcin, and TGF-β1 (p < 0.05, p < 0.01, and p < 0.001, respectively). Cell prolife...Continue Reading

References

Jun 1, 1991·The American Journal of Physiology·J M VanderkooiI A Silver
Oct 31, 1998·Current Opinion in Genetics & Development·G L Semenza
Aug 24, 1999·Plastic and Reconstructive Surgery·D S SteinbrechM T Longaker
Dec 30, 1999·Journal of Leukocyte Biology·J S LewisC E Lewis
Feb 7, 2001·American Journal of Physiology. Cell Physiology·D S SteinbrechM T Longaker
Sep 1, 2000·Science·G A Rodan, T J Martin
Sep 26, 2000·Molecular Genetics and Metabolism·C T D'Angio, J N Finkelstein
Dec 21, 2000·American Journal of Physiology. Cell Physiology·J A SpectorM T Longaker
Jun 26, 2001·The Journal of Surgical Research·S M WarrenM T Longaker
Jan 11, 2002·Blood·Jonathan S HarrisonPersis Bandari
Jun 29, 2002·Molecular and Cellular Endocrinology·J H ParkH S Baek
May 21, 2004·Nature Reviews. Neuroscience·Frank R Sharp, Myriam Bernaudin
Jul 21, 2004·The Journal of Biological Chemistry·Ali SalimMichael T Longaker
Mar 15, 2006·Experimental Cell Research·J C UttingT R Arnett
Jul 13, 2006·Annals of the New York Academy of Sciences·Xuelin Li, Xu Cao
Mar 21, 2007·Journal of Cellular Physiology·Christina M LeeClare E Yellowley
Oct 25, 2007·PloS One·Naohiro KawamuraHiroshi Kawaguchi
Jan 30, 2008·Molecular and Cellular Biochemistry·Jin-Ming HwangWei-Wen Kuo
Feb 12, 2009·Journal of Cell Science·Aditi Mukherjee, Peter Rotwein
Jan 1, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Atsuhiko OikawaPaolo Madeddu
Mar 17, 2010·Journal of Cellular Physiology·Wen-Pei TsengChih-Hsin Tang
Jul 24, 2010·Photomedicine and Laser Surgery·Takeshi KiyosakiNoriyoshi Shimizu
Jul 28, 2010·Lasers in Surgery and Medicine·Keiko FujimotoNoriyoshi Shimizu
Jul 23, 2011·Biochemical and Biophysical Research Communications·You Hee ChoiKwang-Youl Lee

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Citations

Dec 18, 2013·Journal of Photochemistry and Photobiology. B, Biology·Nicolette N HoureldHeidi Abrahamse
Mar 7, 2014·Lasers in Medical Science·Mario MigliarioFilippo Renò
Feb 11, 2014·TheScientificWorldJournal·Nicolette N Houreld
Jul 9, 2013·Lasers in Medical Science·Rodrigo Carvalho Pinto CoelhoJoão Batista Blessmann Weber
Aug 8, 2014·Photomedicine and Laser Surgery·Danilo B HöflingMaria Cristina Chammas
Jan 14, 2016·Veterinary Research Communications·Chiara GomieroJan H Spaas
Jul 21, 2015·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Giovanni MergoniMassimiliano G Bianchi
Oct 9, 2015·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Francisco J Manzano-MorenoConcepción Ruiz
Jan 30, 2015·Journal of Periodontology·Chen-Ying WangPo-Chun Chang
Jul 10, 2013·Lasers in Medical Science·Jae-Yeol LeeSang-Hun Shin
Aug 13, 2013·Lasers in Medical Science·Po-Chun ChangLi Yen Chong
Aug 16, 2016·Journal of Photochemistry and Photobiology. B, Biology·Flávia A OliveiraRodrigo C Oliveira
Jun 12, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Hannah SerrageMichael R Hamblin
Apr 12, 2017·Bioscience Reports·Cong-Xiang JianChen-Jun Li
Oct 18, 2018·Acta Cirúrgica Brasileira·Fernando Russo Costa do BomfimHelio Plapler
Dec 15, 2018·Journal of Biomedical Optics·Randa ZeinMichael R Hamblin
Dec 11, 2019·Materials·Marcelie Priscila de Oliveira RossoRogério Leone Buchaim
Mar 22, 2020·Clinical Oral Investigations·Amanda Miyuki TerayamaLuciano Tavares Angelo Cintra
Dec 21, 2016·Maxillofacial Plastic and Reconstructive Surgery·Sang-Hun ShinCheol-Hun Kim
Feb 6, 2020·Current Stem Cell Research & Therapy·Khatereh KhorsandiReza Fekrazad
Dec 2, 2020·International Journal of Molecular Sciences·Yujin OhsugiTakanori Iwata
Dec 29, 2016·Open Access Macedonian Journal of Medical Sciences·Ahmed Abbas ZakyNoha Mohamed Ismael Awad

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