Low-level laser therapy stimulates mineralization via increased Runx2 expression and ERK phosphorylation in osteoblasts

Photomedicine and Laser Surgery
Takeshi KiyosakiNoriyoshi Shimizu

Abstract

This study examined the effects of low-level laser therapy (LLLT) on osteoblasts via insulin-like growth factor I (IGF-I) signal transduction. Because orthodontic treatment is usually accompanied by bone formation, if bone formation can be promoted, the treatment and retention periods will be shorter. Recently, we reported the stimulatory effects of LLLT on bone formation. It was dependent on increased IGF-I, which plays an essential role in the anabolic regulation of bone metabolism. However, the signal transduction of IGF-I stimulated by LLLT was not elucidated. Mouse osteoblastic MC3T3-E1 cells were cultured with or without LLLT (0.96-3.82 J/cm(2)), and the expression of IGF-I and Runt-related transcription factor 2 (Runx2) and the phosphorylation of extracellular-signal-regulated kinase (ERK) were determined by using real-time PCR and Western blot analysis. LLLT at 1.91 J/cm(2) significantly increased the expression of IGF-I and Runx2 and of ERK phosphorylation. Cyclolignan picropodophyllin (PPP; an IGF-I receptor inhibitor) partly inhibited the LLLT-induced expression of these factors. Moreover, when conditioned medium from the LLLT (1.91 J/cm(2)) cells was added to the MC3T3-E1 culture, the calcium content in the minerali...Continue Reading

References

Dec 1, 1984·Journal of the American Academy of Dermatology·R P AbergelJ Uitto
Feb 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S AmarnaniS Mohan
May 1, 1997·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·S Saito, N Shimizu
Jul 18, 2003·Medical and Pediatric Oncology·Robert C Olney
Dec 4, 2003·Journal of Clinical Laser Medicine & Surgery·Yuji Ueda, Noriyoshi Shimizu
Aug 31, 2004·Lasers in Surgery and Medicine·Delma R CruzNiklaus U Wetter
Apr 7, 2009·Lasers in Surgery and Medicine·Silvia SaracinoGiuliana Muzio
Apr 29, 2009·Lasers in Medical Science·Seyed Kazem ShakouriMohammad Reza Oskuie
Jun 24, 2009·Lasers in Medical Science·Roberto HüblerMarília Gerhardt de Oliveira

❮ Previous
Next ❯

Citations

Jun 15, 2011·Photomedicine and Laser Surgery·Daniela Cervelle ZancanelaAntonio Claudio Tedesco
Oct 3, 2012·Photomedicine and Laser Surgery·Jiaohong DuanYang Zhang
Mar 22, 2013·Lasers in Medical Science·Carla Roberta TimAna Claudia Muniz Rennó
Apr 14, 2015·Periodontology 2000·Akira AokiYuichi Izumi
Jul 15, 2015·The International Journal of Biochemistry & Cell Biology·Jie FengDa Xing
Jan 14, 2016·Iranian Red Crescent Medical Journal·Atarodalsadat MostafaviniaMohammad Bayat
Aug 16, 2016·Journal of Photochemistry and Photobiology. B, Biology·Flávia A OliveiraRodrigo C Oliveira
Feb 17, 2018·Lasers in Medical Science·Giovanni MergoniRita Gatti
Jun 7, 2014·Photomedicine and Laser Surgery·Farzad FazilatAntonio Luiz Barbosa Pinheiro
Jul 21, 2017·Lasers in Medical Science·Mohammadjavad FredoniMohammad Bayat
Oct 18, 2018·Acta Cirúrgica Brasileira·Fernando Russo Costa do BomfimHelio Plapler
Jan 15, 2019·Journal of the Korean Association of Oral and Maxillofacial Surgeons·Seok-Young JeongSang Hun Shin
Jan 12, 2021·Journal of Lasers in Medical Sciences·Devi Bala Raja ThalaimalaiPriyanka K Cholan

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Primer3
Smart Cycler

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.