Abstract
Objectives: Low-level laser therapy (LLLT) is a promising non-invasive treatment option for oropharyngeal mucositis, which is a common side effect of many oncological treatments. LLLT is known for its wound healing properties due to the stimulation of cellular processes, such as proliferation, migration and differentiation. Controversy exists on the possible stimulatory effect of LLLT on head and neck cancer (HNSCC) cells in patients treated with radiotherapy. The aim of this study was to evaluate the biostimulatory effect together with the underlying mechanisms of LLLT on HNSCC cancer cells and normal epithelial cells. Materials and methods: HNSCC cell lines (SCC154, SQD9, and SCC61) and human tonsil epithelial cells were exposed to a Gallium-Aluminum-Arsenide diode laser (830 nm, 150 mW) with energy densities of 0, 1, and 2 J/cm2. The proliferation potential of the cells was assessed by Sulforhodamine B assay, immunoblotting (mitogenic pathways), immunocytochemistry (Ki67), and flow cytometry (PI cell cycle staining). Results: Cell proliferation was increased in HNSCC cell lines after laser irradiation with 1 J/cm2, whereas no significant increase was seen after laser irradiation with 2 J/cm2. In contrast, no effect on cell p...Continue Reading
References
Mar 10, 2001·Journal of Cellular Physiology·G SheferO Halevy
May 14, 2003·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Andy TrottiMarya D Zilberberg
Apr 22, 2005·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·William PostenMurad Alam
Sep 13, 2005·Journal of Cellular Physiology·Xuejuan GaoXunbin Wei
Dec 17, 2005·Photomedicine and Laser Surgery·Jurema Lisboa Freire de CastroCristina Pacheco Soares
Jan 15, 2009·Journal of Cellular Physiology·Lingling ZhangShengnan Wu
Jan 29, 2011·Lasers in Medical Science·Khalid M AlGhamdiNoura A Moussa
Feb 23, 2011·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Volker Hans SchartingerJózsef Dudás
Jul 2, 2011·Photomedicine and Laser Surgery·Juliana S de C MonteiroJean Nunes Dos Santos
Apr 5, 2013·Journal of Biophotonics·Felipe F SperandioSuzana C O M de Sousa
Sep 21, 2013·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Heliton S AntunesCarlos G Ferreira
Jan 30, 2014·Cancer Research·Rüveyda DokSandra Nuyts
Feb 15, 2014·Lasers in Medical Science·Águida Cristina Gomes HenriquesRoseana de Almeida Freitas
Aug 27, 2014·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·K Kian AngRita S Axelrod
Feb 24, 2015·Journal of Photochemistry and Photobiology. B, Biology·Ajay Prashad GautamVausudev Guddattu
Mar 18, 2016·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Judith A E M ZechaRené-Jean Bensadoun
Mar 18, 2016·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Judith A E M ZechaRené-Jean Bensadoun
May 20, 2016·Photomedicine and Laser Surgery·Fernanda Aurora Stabile GonnelliHelena Regina Comodo Segreto
Aug 20, 2016·Lasers in Medical Science·Jolien RobijnsJeroen Mebis
Mar 5, 2017·Lasers in Medical Science·Luiz Felipe PalmaHelena Regina Comodo Segreto
Mar 13, 2017·Lasers in Medical Science·Fernanda Gonçalves BassoCarlos Alberto de Souza Costa
May 4, 2017·Journal of Biophotonics·Gholamreza Esmaeeli DjavidMichael R Hamblin
Jul 10, 2017·Oral Oncology·Héliton S AntunesCarlos G Ferreira
Oct 12, 2017·Journal of Cosmetic and Laser Therapy : Official Publication of the European Society for Laser Dermatology·Wilfredo Alejandro González-ArriagadaMarcio Ajudarte Lopes
Nov 22, 2017·Cell Proliferation·C KaraN Kara
Citations
Mar 1, 2019·The Journal of Craniofacial Surgery·Jason J YooSeth R Thaller
Aug 20, 2020·Lasers in Medical Science·Ana Melissa Ccopa IbarraMaria Fernanda Setúbal Destro Rodrigues
Jun 19, 2019·Lasers in Medical Science·Jéssica Lúcio da SilvaLuciana Prado Maia
May 29, 2020·Lasers in Medical Science·Ye TianHyun Wook Kang
Sep 11, 2019·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·M T Genot-KlasterskyJ A Klastersky
Nov 14, 2019·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Stephen Sonis
Jun 26, 2020·Lasers in Medical Science·Rene FischlechnerHerbert Riechelmann
Aug 15, 2020·Frontiers in Oncology·Shing Yau TamShara W Y Lee
Jul 28, 2020·Cancers·Reem HannaIoana Roxana Bordea
Oct 5, 2020·Photochemistry and Photobiology·Saulo T PereiraMartha S Ribeiro
Oct 28, 2020·Cancer Medicine·René-Jean BensadounUNKNOWN World Association for Laser Therapy (WALT)
Dec 12, 2020·Photodiagnosis and Photodynamic Therapy·Maedeh YousefiAhmadreza Shamshiri
Apr 4, 2021·Cancers·Hyejin KimHyun Wook Kang
May 1, 2021·BMC Oral Health·Elodie CourtoisNicolas Magné
May 7, 2021·PloS One·Afsaneh EbrahiminaseriZohreh Safari
Jun 18, 2021·Heliyon·Rosane de Fátima Zanirato LizarelliVanderlei Salvador Bagnato
Nov 5, 2021·Frontiers in Oncology·Yuqi XiaDi Zheng