Low-molecular-weight displacers for high-resolution protein separations

Analytical Biochemistry
A Kundu, Steven M Cramer

Abstract

The resolving power of displacement chromatography using low-molecular-weight displacers was investigated using a model mixture containing bovine and horse heart cytochrome c. The linear and nonlinear adsorption behavior of these two proteins was examined in cation-exchange chromatography and shown to be quite similar. Furthermore, an analysis of the dynamic affinity of these proteins indicated extremely similar affinities under displacement conditions. Despite the extreme similarities in the adsorption behavior, displacement chromatography using a protected amino acid displacer resulted in excellent separation of the proteins with both high yields and purity. These results indicate that displacement chromatography may be efficacious for a wide variety of difficult protein separation problems.

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Citations

Sep 14, 2010·Journal of Chromatography. a·Christopher J MorrisonSteven M Cramer
Jun 6, 2009·Biotechnology Progress·Christopher J Morrison, Steven M Cramer
Nov 26, 2008·Biotechnology and Bioengineering·Christopher J MorrisonSteven M Cramer
Feb 11, 2005·Journal of Chromatography. a·N Tugcu, S M Cramer
Jan 5, 2010·Journal of Chromatography. a·Steven T EvansSteven M Cramer
Apr 12, 2002·Biotechnology and Bioengineering·V NatarajanS M Cramer
Aug 8, 2006·Biotechnology Journal·Klaus Graumann, Andreas Premstaller
Feb 25, 2003·Biotechnology and Bioengineering·Khurram M SunasaraSteven M Cramer
Jun 3, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Srinavya VutukuruRavi S Kane
Sep 16, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Srinavya Vutukuru, Ravi S Kane
Dec 5, 2008·Journal of the American Chemical Society·Christopher J MorrisonJ A Moore
Mar 27, 2010·Analytical Chemistry·Christopher J MorrisonSteven M Cramer
Apr 29, 2011·Analytical Chemistry·Steven T EvansSteven M Cramer
Nov 1, 2005·Analytical Chemistry·Kaushal RegeSteven M Cramer

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