Low-Molecular-Weight Fucoidan Inhibits the Viability and Invasiveness and Triggers Apoptosis in IL-1β-Treated Human Rheumatoid Arthritis Fibroblast Synoviocytes

Inflammation
Zunhua ShuBingyu Guan

Abstract

Fucoidan is a sulfated polysaccharide found mainly in various species of brown algae and brown seaweed. Here, we investigated the effects of low-molecular-weight (LMW) fucoidan (4 kDa) on interleukin-1beta (IL-1β)-stimulated rheumatoid arthritis fibroblast-like synoviocyte (RAFLS). 3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay and annexin V/propidium iodide assay were used to assess cell viability and apoptosis, respectively. Transwell assay was performed to evaluate cell invasion. Reverse transcription polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assay analysis was done to measure gene expression and secretion. Nuclear factor-kappa B (NF-κB) DNA binding activity was determined by electrophoretic mobility shift assay. LMW fucoidan dose-dependently inhibited the viability and induced apoptosis of IL-1β-treated RAFLS. Fucoidan attenuated IL-1β-induced invasion of RAFLS and decreased the expression and secretion of metalloproteinase (MMP)-1, MMP-3, and MMP-9. Fucoidan suppressed NF-κB binding activity, p65 nuclear translocation, and IκB-α degradation in IL-1β-stimulated RAFLS. Additionally, IL-1β-induced phosphorylation of p38 but not ERK or JNK was significantly impaired by fucoid...Continue Reading

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Citations

Jan 31, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Victor FattoriWaldiceu A Verri
Aug 3, 2016·Marine Drugs·Lucas CholletFrédéric Chaubet
Apr 7, 2017·Folia medica·Maria H KazakovaVictoria S Sarafian
Jan 24, 2020·Critical Reviews in Food Science and Nutrition·Benwei ZhuZhong Yao
Feb 18, 2021·Carbohydrate Polymers·Carlos Vaamonde-GarcíaRosa Meijide-Faílde
May 25, 2020·International Journal of Biological Macromolecules·Ramar ManikandanNarayanasamy Marimuthu Prabhu

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis