PMID: 2511038Oct 1, 1989Paper

Low molecular weight iron from guinea pig reticulocytes isolated by Sephadex G-25 chromatography

European Journal of Haematology
J WeaverH Zhan

Abstract

As part of a continuing study of the low MW iron pool, guinea pig reticulocytes were incubated with 59Fe-labeled transferrin, and the reticulocyte hemolysate was chromatographed on Sephadex G-25. 59Fe, in amounts corresponding to that which was in a low MW peak eluting from an Ultrogel column and to that not precipitated by ammonium sulfate, adsorbed to the Sephadex column. The adsorbing 59Fe, on elution from the Sephadex with dilute formic acid, coeluted with phosphate and pentose. When EDTA was added to disrupt the putative iron complex, neither iron nor P adsorbed to the column, supporting the argument that they exist as a compound in the cytosol and adsorb and elute together for that reason. These observations provide additional evidence that P-containing compounds, probably originating as nucleotides, are important components of the low MW iron pool of cells.

References

Dec 7, 1976·Ciba Foundation Symposium·A Jacobs
May 1, 1985·American Journal of Hematology·S PollackJ Weaver
Jan 1, 1986·The International Journal of Biochemistry·M MulliganM C Linder
Sep 14, 1973·Biochimica Et Biophysica Acta·N L MeyersF J Oelshlegel
Apr 1, 1971·British Journal of Haematology·D A LipschitzR W Charlton
Oct 1, 1982·British Journal of Haematology·M J PippardC A Finch
Oct 7, 1964·Annals of the New York Academy of Sciences·H KEBERLE

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Citations

Feb 22, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Maya Shvartsman, Z Ioav Cabantchik
Jan 1, 1994·Biological Trace Element Research·L J AnghileriJ F Escanero
Apr 4, 2007·Neurochemical Research·Julie A GaaschCornelis J Van der Schyf
Jun 18, 2021·Seminars in Cancer Biology·Peter W HalcrowJoyce E Ohm

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