PMID: 20132736Feb 6, 2010Paper

Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells

BMB Reports
Jwa-Young KimJoo-Hong Yeo

Abstract

Silk fibroin, produced by the silkworm Bombyx mori, has been widely studied as a scaffold in tissue engineering. Although it has been shown to be slowly biodegradable, cellular responses to degraded silk fibroin fragments are largely unknown. In this study, silk fibroin was added to MG-63 cell cultures, and changes in gene expression in the MG-63 cells were screened by DNA microarray analysis. Genes showing a significant (2-fold) change were selected and their expression changes confirmed by quantitative RT-PCR and western blotting. DNA microarray results showed that alkaline phosphatase (ALP), collagen type-I alpha-1, fibronectin, and transforming growth factor-beta1 expressions significantly increased. The effect of degraded silk fibroin on osteoblastogenic gene expression was confirmed by observing up-regulation of ALP activity in MG-63 cells. The finding that small fragments of silk fibroin are able to increase the expression of osteoblastogenic genes suggests that controlled degradation of silk fibroin might accelerate new bone formation. [BMB reports 2010; 43(1): 52-56].

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Citations

Jan 30, 2013·Chinese Journal of Cancer Research = Chung-kuo Yen Cheng Yen Chiu·Yan RenPing-Ping Li
Sep 23, 2014·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·HaeYong KweonSeong-Gon Kim
Jan 29, 2011·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·HaeYong KweonSeong-Gon Kim
Oct 20, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Dohiko TeradaYasushi Tamada
Aug 23, 2011·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Ji-Young SongJwa-Young Kim
Jul 9, 2014·ACS Applied Materials & Interfaces·Christian B BorknerThomas Scheibel
Apr 20, 2017·International Journal of Molecular Sciences·You-Young JoHyun Seok
Nov 1, 2014·Maxillofacial Plastic and Reconstructive Surgery·Sang-Woon Lee, Seong-Gon Kim
Apr 14, 2016·Maxillofacial Plastic and Reconstructive Surgery·Seong-Gon KimJeong Keun Lee
Jun 7, 2018·Maxillofacial Plastic and Reconstructive Surgery·Ju-Won KimSeong-Gon Kim
Jul 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·So-Young ParkJun Hong Park

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