Low molecular weight xanthan gum suppresses oxidative stress-induced apoptosis in rabbit chondrocytes

Carbohydrate Polymers
Qixin ChenFengshan Wang

Abstract

We have previously reported the application of low molecular weight XG(LM-XG), with molecular weights ranging from 1×106Da to 1.5×106Da for treating osteoarthritis. In this study, we investigated the anti-apoptotic activity of LM-XG under oxidative stress conditions, activated by hydrogen peroxide (H2O2)-treated chondrocytes in vitro. Chondrocytes were pretreated with various doses of LM-XG (0, 10, 100, 500, or 1000μg/mL) or 1000μg/mL sodium hyaluronate for 12h, and then exposed to 0.5mmol/L H2O2 for another 12h. After treatment, chondrocyte viability was evaluated using a cell counting kit-8; DNA fragmentation was detected using Hoechst33258 staining; the percentage of DNA fragmentation was evaluated using the diphenylamine DNA assay kit; the apoptosis rate was evaluated using flow cytometry; chondrocyte ultra-microscopic morphology was observed using transmission electron microscopy; intracellular reactive oxygen species levels were observed and quantified using 2,7-dichlorofuorescin diacetate, mitochondrial permeability transition analysis was performed using MitoTracker Red CMXRos and 4',6-diamidino-2-phenylindole staining; and finally, caspase-3 activity was detected by western blot. The results showed that, compared with ...Continue Reading

Citations

Oct 8, 2019·The Journal of Reproduction and Development·Yasuhisa MunakataHisataka Iwata
Jun 21, 2020·Reproduction in Domestic Animals = Zuchthygiene·Shunsuke HaraHisataka Iwata
Jan 3, 2018·International Journal of Molecular Sciences·Sara CheleschiAntonella Fioravanti
Dec 17, 2020·Frontiers in Medicine·Xingjia MaoChuan Xiang

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis