Low Na+ diet inhibits Na+ and water transport response to vasopressin in rat cortical collecting duct

Kidney International
J A Schafer, L Chen

Abstract

We previously demonstrated that vasopressin (AVP) produces a sustained increase in Na+ reabsorption by the isolated perfused cortical collecting duct (CCD) from rats on a normal diet, and that this effect is synergistic with that of pharmacological doses of deoxycorticosterone (DOC) or physiological levels of aldosterone. The present experiments examined the effect of AVP under the more physiological circumstances when plasma aldosterone was elevated by prior volume depletion. Rats were volume depleted by a single dose of furosemide followed by a low-salt diet (0.3% NaCl) for four to nine days. Some of these rats were also implanted with a pellet containing 2.5 mg DOC. Rats in a third group were not injected with furosemide but were implanted with the DOC pellet and maintained on a standard (approximately 1% NaCl) diet. CCD were perfused and the lumen-to-bath Na+ flux (JNA), transepithelial voltage (VT), and osmotic water permeability (Pf) were measured in the presence and absence of 200 pm AVP. Although Na+ depletion by a single injection of furosemide and the low salt diet elevated plasma aldosterone and Vt, JNA remained low and there was a decreased response to AVP in comparison with DOC-treated rats on a standard diet. In C...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·J C Williams, J A Schafer
Apr 1, 1986·The Journal of Clinical Investigation·M C ReifJ A Schafer
Apr 1, 1985·The Journal of Clinical Investigation·B StantonJ Wade
Aug 1, 1971·The American Journal of Physiology·F S WrightG Giebisch
Dec 1, 1981·The Journal of Clinical Investigation·K J PettyD Marver
Sep 1, 1983·Kidney International·R S MartinJ P Hayslett
Jul 1, 1996·Kidney International·M D PlotkinS C Hebert

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Citations

May 9, 2009·Journal of the American Society of Nephrology : JASN·Scott S P WildmanRobert J Unwin
Nov 14, 2014·American Journal of Physiology. Renal Physiology·Elena MironovaJames D Stockand
Aug 20, 2003·The Journal of Clinical Investigation·Isabelle RuberaBernard C Rossier
Jul 20, 2006·American Journal of Physiology. Renal Physiology·Peng SunWen-Hui Wang

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