Low oxygen tension inhibits osteogenic differentiation and enhances stemness of human MIAMI cells

Bone
Gianluca D'IppolitoPaul C Schiller

Abstract

We recently reported the isolation of a unique subpopulation of human stromal cells from bone marrow (BM) termed marrow-isolated adult multilineage inducible (MIAMI) cells, capable of differentiating in vitro into mature-like cells from all three germ layers. The oxygen tension (pO2) in BM ranges from 1 to 7%, which prompted us to examine the role of pO2 in regulating the capacity of MIAMI cells both to self-renew and maintain their pluripotentiality (stemness) or to progress toward osteoblastic differentiation. MIAMI cells were grown under low-pO2 conditions (1, 3, 5, and 10% oxygen) or air (21% oxygen). The proliferation rate of cells exposed to 3% oxygen (3 days) increased, resulting in cell numbers more than threefold higher than those of cells exposed to air (at 7 days). In cells grown under osteoblastic differentiation conditions, the expression of the osteoblastic markers osteocalcin, bone sialoprotein, osterix, and Runx2 and alkaline phosphatase activity was upregulated when incubated in air; however, it was blocked at low (3%) pO2. Similarly, biomineralization of long-term cell cultures was high under osteoblastic differentiation conditions in air but was undetectable at low (3%) pO2. In contrast, low pO2 upregulated m...Continue Reading

References

Nov 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C T BrightonC C Clark
Oct 1, 1985·Acta Orthopaedica Scandinavica·H KofoedH P Olesen
Dec 29, 1995·The Journal of Biological Chemistry·A NamikiJ M Isner
Aug 1, 1996·Journal of Cellular Physiology·R RajpurohitI M Shapiro
Sep 12, 1997·Science·O GenbacevS J Fisher
May 30, 2001·Trends in Molecular Medicine·A I Caplan, S P Bruder
Jun 29, 2002·Molecular and Cellular Endocrinology·J H ParkH S Baek
Sep 13, 2002·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Till Acker, Karl H Plate
Oct 26, 2002·Free Radical Biology & Medicine·Carine MichielsMartine Raes
May 3, 2003·Cancer Cell·Selma PennacchiettiPaolo M Comoglio
Jul 12, 2003·Nature Cell Biology·Simona ParrinelloJudith Campisi
Jul 17, 2003·Journal of Hematotherapy & Stem Cell Research·Denis English
Aug 2, 2003·Blood·Erica L HerzogDiane S Krause
May 1, 1963·Annals of Internal Medicine·J L GRANT, B SMITH
Mar 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Toshihiko EzashiR Michael Roberts

❮ Previous
Next ❯

Citations

Sep 23, 2008·Wiener medizinische Wochenschrift·Pamina PflegerlLukas Kenner
Jul 27, 2007·Lab on a Chip·Jaesung ParkMartin L Yarmush
Jul 14, 2007·Antioxidants & Redox Signaling·Keith A Webster
Sep 25, 2008·Stem Cells and Development·Suzanne D WestfallToshihiko Ezashi
Apr 12, 2013·Stem Cells and Development·Mey Al-HabibGeorge T-J Huang
May 23, 2007·Tissue Engineering·Jos MaldaZee Upton
Feb 7, 2009·Tissue Engineering. Part a·Deborah SchopRiemke van Dijkhuizen-Radersma
Aug 24, 2011·Tissue Engineering. Part a·Debby GawlittaWouter J A Dhert
Apr 26, 2011·Tissue Engineering. Part a·Natalja E FedorovichJacqueline Alblas
Sep 29, 2011·Tissue Engineering. Part a·Wayne Yuk Wai LeeYun Feng Rui
Dec 12, 2012·Tissue Engineering. Part a·Shimon BenjaminZulma Gazit
Feb 12, 2013·Tissue Engineering. Part a·Suchit SahaiJames O Blanchette
Feb 6, 2010·Tissue Engineering. Part B, Reviews·Debby GawlittaWouter J A Dhert
Jan 21, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yun SunXiao-Dong Chen
Jul 28, 2013·Fiziologiia cheloveka·E R andreeva, L B Buravkova
Jul 28, 2010·American Journal of Physiology. Cell Physiology·Adam M ZahmKarthik Mukundakrishnan
Mar 15, 2011·BMC Developmental Biology·Eric W BrunskillLarry T Patterson
Aug 24, 2012·BMC Veterinary Research·Beatriz RaneraClementina Rodellar
Feb 22, 2012·International Journal of Nanomedicine·Vicente Torres-CostaRaúl J Martín-Palma
Jan 24, 2013·International Journal of Molecular Sciences·Ina Berniakovich, Marco Giorgio
May 25, 2011·World Journal of Stem Cells·Farbod RastegarTong-Chuan He
Nov 6, 2014·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·M V Pogodina, L B Buravkova
Oct 24, 2013·Regenerative Medicine·Kate E HawkinsTristan R McKay
Nov 20, 2014·Cell Regeneration·Guangming Wu, Hans R Schöler
May 27, 2014·Injury·K D HankensonR Marcucio

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.