PMID: 2497783May 19, 1989Paper

Low-pH association of proteins with the membranes of intact red blood cells. II. Studies of the mechanism

Biochimica Et Biophysica Acta
T ArvinteC Nicolau

Abstract

The low-pH interaction of proteins with erythrocyte membranes has been found to be correlated with pH-induced changes in the erythrocyte membrane. Using a 90 degree lightscattering method it was shown that red blood cell hemolysis was slow between pH 5.8 and 5 (t1/2 above 1 h) but became fast at and below pH 4.7 (t1/2 less than 20 min). At pH 4.7, the presence of glycophorin in the incubation medium inhibited the hemolysis of erythrocytes and this protective effect was found to be dependent on the glycophorin concentration. Electron microscope experiments showed the presence of membrane defects after 10 s incubation at pH 4.6 in the absence of glycophorin in the incubation medium. These defects could further develop into openings with average widths of 14 nm after 1.5 min incubation under the acidic conditions. Fluorescence and flow cytometry studies showed that at pH 4.7, but not at pH 7.4, glycophorin tightly associates with phosphatidylcholine liposomes, and that liposome associated glycophorin molecules are recognized by anti-glycophorin monoclonal antibodies.

References

Jun 2, 1978·Biochimica Et Biophysica Acta·B de KruijffL L van Deenen
May 16, 1978·European Journal of Biochemistry·E J van ZoelenL L van Deenen
Dec 4, 1978·Biochimica Et Biophysica Acta·E J van ZoelenL L van Deenen
Apr 27, 1987·Biochimica Et Biophysica Acta·M K Jain, D Zakim
Jun 9, 1988·Biochimica Et Biophysica Acta·G von Heijne
Feb 23, 1988·Biochemistry·L A Chung, E London
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D M Haverstick, M Glaser
Dec 1, 1974·The Journal of Cell Biology·A Elgsaeter, D Branton
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B L KaganM Colombini
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J J SkehelD C Wiley
Sep 1, 1955·Archives of Biochemistry and Biophysics·E HUTCHINSON, K E BEAN
Mar 26, 1956·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·E RIDEAL, F H TAYLOR
Oct 1, 1950·Journal of Cellular Physiology·L H LOVE

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.