Low pH-induced changes in Pseudomonas exotoxin and its domains: increased binding of Triton X-114.

Infection and Immunity
T IdziorekI Pastan

Abstract

Pseudomonas exotoxin (PE), which is composed of three structural domains, is a 66-kilodalton protein secreted by P. aeruginosa that is cytotoxic for mammalian cells. After binding to cell surface receptors and internalization into low-pH endocytic vesicles, PE or an active fragment kills mammalian cells by translocating across an intracellular membrane to the cytoplasm and shutting down protein synthesis. To investigate possible conformational changes associated with the translocation process, full-length PE or recombinant proteins containing the PE cell recognition domain, translocation domain, enzymatic domain, or translocation plus enzymatic domains were incubated with Triton X-114 at pH values ranging from 3.0 to 7.0. The truncated forms used were intact domains that had been expressed in Escherichia coli and subsequently purified. Previous studies (K. Sandvig and J. O. Moskaug, Biochem. J. 245:899-901, 1987) had shown that full-length PE bound more Triton X-114 at a low pH than at a physiologic pH. Therefore, we investigated whether this increased binding was due to a global change in PE or a change within a particular domain. Results showed that all the truncated toxin proteins displayed a similar pH-dependent entry into ...Continue Reading

References

Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·B H Iglewski, D Kabat
Jan 1, 1988·Cancer Treatment and Research·S Olsnes, K Sandvig
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·V S AlluredD B McKay
Aug 1, 1987·The Biochemical Journal·K Sandvig, J O Moskaug
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·V K ChaudharyI Pastan
Dec 1, 1985·Infection and Immunity·L S Zalman, B J Wisnieski
Dec 1, 1965·Journal of Bacteriology·K Kim, N B Groman
Jan 1, 1984·Experimental Cell Research·M J Geisow, W H Evans
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G L GrayH L Heyneker
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L GreenfieldD A Kaplan
Sep 1, 1984·Microbiological Reviews·J L Middlebrook, R B Dorland
Nov 1, 1983·Reviews of Infectious Diseases·D FitzGeraldC B Saelinger
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle

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Citations

Nov 17, 1995·The Journal of Biological Chemistry·D TortorellaE London
Jan 1, 1994·Critical Reviews in Biochemistry and Molecular Biology·A Sánchez-FerrerF García-Carmona
Mar 26, 1992·Biochimica Et Biophysica Acta·E London

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