Low-picomolar limits of detection using high-power light-emitting diodes for fluorescence

The Analyst
Ebbing P de Jong, Charles A Lucy

Abstract

Fluorescence detectors are ever more frequently being used with light-emitting diodes (LEDs) as the light source. Technological advances in the solid-state lighting industry have produced LEDs which are also suitable tools in analytical measurements. LEDs are now available which deliver 700 mW of radiometric power. While this greater light power can increase the fluorescence signal, it is not trivial to make proper use of this light. This new generation of LEDs has a large emitting area and a highly divergent beam. This presents a classic problem in optics where one must choose between either a small focused light spot, or high light collection efficiency. We have selected for light collection efficiency, which yields a light spot somewhat larger than the emitting area of the LED. This light is focused onto a flow cell. Increasing the detector cell internal diameter (i.d.) produces gains in (sensitivity)3. However, since the detector cell i.d. is smaller than the LED spot size, scattering of excitation light towards the detector remains a significant source of background signal. This can be minimized through the use of spectral filters and spatial filters in the form of pinholes. The detector produced a limit of detection (LOD)...Continue Reading

References

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Aug 1, 2005·Analytica Chimica Acta·Ebbing P de Jong, Charles A Lucy

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Citations

Aug 17, 2012·Analytical and Bioanalytical Chemistry·I M Perez de Vargas-SansalvadorL F Capitan-Vallvey
May 14, 2014·Annual Review of Analytical Chemistry·Mirek MackaPurnendu K Dasgupta
Jun 5, 2012·Journal of Environmental Sciences (China)·Shuhua XueHai-Fang Li
Nov 27, 2014·Analytical Chemistry·Jan Prikryl, Frantisek Foret
Oct 30, 2007·Journal of Chromatography. a·Paul R HaddadWolfgang Buchberger
Feb 24, 2007·Bioorganic Chemistry·Matthew NoesthedenJohn Paul Pezacki
Dec 17, 2008·Electrophoresis·Dan XiaoMartin M F Choi
Jan 4, 2012·Electrophoresis·Véréna PoinsotFrançois Couderc
Oct 29, 2010·The Analyst·Łukasz TymeckiRobert Koncki
May 23, 2008·Analytical Chemistry·Mark LowryIsiah M Warner
Jul 13, 2021·Analytica Chimica Acta·Hai Yen TaFrancois Couderc

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