Low-potency glucocorticoid hydrocortisone has similar neurotoxic effects as high-potency glucocorticoid dexamethasone on neurons in the immature chicken cerebellum

Brain Research
Petra AdenJon Lømo

Abstract

High-potency glucocorticoids (GC) are used in the prophylaxis and treatment of neonatal bronchopulmonal dysplasia, but there is concern about side effects on the developing brain. Recently, the low-potency GC hydrocortisone (HC) has been suggested as an alternative to high-potency GC. We compared the neurotoxic effects of HC with the high-potency GC dexamethasone (DEX) in chicken cerebellum. A single dose of GC was injected into the egg at embryonic day 16 and the death of granule neurons in histologic sections of the cerebellar cortex was examined 24 h later. DEX and HC showed a similar dose-dependent induction of morphological apoptosis and caspase-3 activation in the internal granular layer. A doubling of the apoptosis rate compared to the basal rate was seen for the highest dose of DEX (5 mg/kg) and medium dose of HC (1 mg/kg). In cultures of embryonic chicken cerebellar granule cells, DEX and HC increased cell death and induced rapid caspase-3 activation in a similar dose-dependent manner. Transfection of granule cells with a luciferase reporter gene revealed that the dose needed for the activation of gene transcription (classical signalling pathway) with DEX was much lower than for HC. In conclusion, HC does not present i...Continue Reading

References

Dec 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·V Hamburger, H L Hamilton
May 1, 1990·Brain Research. Developmental Brain Research·H UnoP M Farrell
Aug 1, 1970·The Journal of Comparative Neurology·P Rakic, R L Sidman
Jan 1, 1994·Rheumatology International·H TanakaI Makino
Aug 1, 1997·Journal of Chemical Neuroanatomy·L P Reagan, B S McEwen
Oct 21, 2000·Archives of Disease in Childhood. Fetal and Neonatal Edition·E S ShinwellE Gottfried
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·E AhlbomS Ceccatelli
Jun 21, 2001·Pediatric Pulmonology·R J BoltH A Delemarre-van de Waal
Mar 9, 2002·Biochemical and Biophysical Research Communications·Hege H SlagsvoldRagnhild E Paulsen
Oct 11, 2003·Journal of Clinical Pharmacology·Donald E MagerWilliam J Jusko
Mar 27, 2004·The New England Journal of Medicine·Tsu F YehCheng H Tsai
Mar 28, 2006·Journal of Neuroscience Methods·Chris M JacobsRagnhild E Paulsen
Mar 27, 2007·The Journal of Pediatrics·Karin J RademakerLinda S de Vries
Jul 17, 2007·Molecular and Cellular Endocrinology·Cindy StahnFrank Buttgereit
Sep 21, 2007·The New England Journal of Medicine·Caroline A CrowtherUNKNOWN ACTORDS Study Group
Sep 21, 2007·The New England Journal of Medicine·Ronald J WapnerUNKNOWN National Institute of Child Health and Human Development Maternal-Fetal Medicine Units Network

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Citations

Jul 12, 2013·Neuroradiology·Emily W Y Tam
Oct 21, 2011·Science Translational Medicine·Emily W Y TamSteven P Miller
Apr 25, 2012·Neurology Research International·Valerie BiranDonna M Ferriero
May 30, 2013·Brain Structure & Function·Maren C KiesslingChristoph Schmitz
Feb 25, 2010·The Journal of Obstetrics and Gynaecology Research·Sumire YamateHideo Nishigori
Dec 27, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Iwona Inkielewicz-StepniakMichal Wozniak
Oct 26, 2016·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Lars Peter Engeset AustdalRagnhild E Paulsen
Jan 1, 2012·Journal of Functional Biomaterials·Susana RodriguesAna Grenha
Sep 20, 2018·Birth Defects Research·Meredith M HowleyUNKNOWN National Birth Defects Prevention Study
Feb 25, 2018·Archives of Disease in Childhood. Fetal and Neonatal Edition·Linan ZengLingli Zhang
Oct 4, 2015·The Journal of Pharmacology and Experimental Therapeutics·Sigrid BjørnstadRagnhild Elisabeth Paulsen
Jan 22, 2021·The Cerebellum·Adaze Bijou Enogieru, Oghenakhogie Iroboudu Momodu
Sep 22, 2012·The Journal of Experimental Biology·Elena SivukhinaMarie-Pierre Moisan
Jun 29, 2021·Frontiers in Systems Neuroscience·Giulia SpotoGabriella Di Rosa
Nov 3, 2021·Pediatric Research·Buse Özer BekmezEsra Çakır

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