Low-protein diet disrupts the crosstalk between the PKA and PKC signaling pathways in isolated pancreatic islets

The Journal of Nutritional Biochemistry
Bruno Rodrigo da Silva LippoFabiano Ferreira

Abstract

Protein restriction in the early stages of life can result in several changes in pancreatic function. These alterations include documented reductions in insulin secretion and in cytoplasmic calcium concentration [Ca(2+)]i. However, the mechanisms underlying these changes have not been completely elucidated and may result, in part, from alterations in signaling pathways that potentiate insulin secretion in the presence of glucose. Our findings suggest that protein restriction disrupts the insulin secretory synergism between Cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) and Ca(2+)-dependent protein kinase C (PKC) in isolated islets. Western blot analysis demonstrated reduced levels of both phospho-cAMP response element-binding protein (phospho-CREB) at Ser-133 and substrates phosphorylated by PKCs (Phospho-(Ser) PKC substrate), suggesting that PKA and PKC activity was impaired in islets from rats fed a low-protein diet (LP). cAMP levels and global Ca(2+) entry were also reduced in LP islets. In summary, our findings showed that protein restriction altered the crosstalk between PKA and PKC signaling pathways, resulting in the alteration of secretory synergism in isolated islets.

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Citations

Jan 7, 2016·Molecular and Cellular Endocrinology·Niyati MalkaniMadhulika B Gupta
Sep 17, 2016·Scientific Reports·Nayara Carvalho LeiteEverardo Magalhães Carneiro
Apr 4, 2017·Journal of Cellular Physiology·Ana Paula G CappelliEverardo M Carneiro
May 20, 2020·Clinical Medicine Insights. Endocrinology and Diabetes·Andrew English, Nigel Irwin
Jul 28, 2021·Molecular and Cellular Endocrinology·Allan W ChenMadhulika B Gupta

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